Genetically Engineered Protein-Based Bioadhesives with Programmable Material Properties

Juya Jeon; Kok Zhi Lee; Xiaolu Zhang; John Jaeger; Eugene Kim; Jingyao Li; Larisa Belaygorod; Batool Arif; Guy M. Genin; Marcus B. Foston; Mohamed A. Zayed; Fuzhong Zhang

doi:10.1021/acsami.3c12919

journal article·2023-12-01Open access

Genetically Engineered Protein-Based Bioadhesives with Programmable Material Properties

Juya Jeon, Kok Zhi Lee, Xiaolu Zhang, John Jaeger, Eugene Kim, Jingyao Li, Larisa Belaygorod, Batool Arif, Guy M. Genin, Marcus B. Foston, Mohamed A. Zayed, Fuzhong Zhang

ACS Applied Materials & Interfaces

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Source abstract

Abstract

Silk-amyloid-mussel foot protein (SAM) hydrogels made from recombinant fusion proteins containing β-amyloid peptide, spider silk domain, and mussel foot protein (Mfp) are attractive bioadhesives as they display a unique combination of tunability, biocompatibility, bioabsorbability, strong cohesion, and underwater adhesion to a wide range of biological surfaces. To design tunable SAM hydrogels for tailored surgical repair applications, an understanding of the relationships between protein sequence and hydrogel properties is imperative. Here, we fabricated SAM hydrogels using fusion proteins of varying lengths of silk-amyloid repeats and Mfps to characterize their structure and properties. We found that increasing silk-amyloid repeats enhanced the hydrogel’s β-sheet content ( r = 0.74), leading to higher cohesive strength and toughness. Additionally, increasing the Mfp length beyond the half-length of the full Mfp sequence (1/2 Mfp) decreased the β-sheet content ( r = −0.47), but increased hydrogel surface adhesion. Among different variants, the hydrogel made of 16xKLV-2Mfp displayed a high ultimate strength of 3.0 ± 0.3 MPa, an ultimate strain of 664 ± 119%, and an attractive underwater adhesivity of 416 ± 20 kPa to porcine skin. Collectively, the sequence-structure–property relationships learned from this study will be useful to guide the design of future protein adhesives with tunable characteristics for tailored surgical applications.

Source: openalex_inverted_index · Quality: baseline

Research context

Polymers Biomaterials

OpenAlex topics: Silk-based biomaterials and applications · Electrospun Nanofibers in Biomedical Applications · 3D Printing in Biomedical Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United States

Department of Surgery, Section of Vascular Surgery, Washington University of Medicine in St. Louis, 660 South Euclid Avenue, Saint Louis, Missouri 63110, United States

NSF Science and Technology Center for Engineering MechanoBiology, Department of Mechanical Engineering & Materials Science, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United States

Institute of Materials Science and Engineering, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United States

Division of Biological & Biomedical Sciences, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United States

Department of Radiology, Washington University of Medicine in St. Louis, 660 South Euclid Avenue, Saint Louis, Missouri 63110, United States

Division of Molecular Cell Biology, Washington University of Medicine in St. Louis, 660 South Euclid Avenue, Saint Louis, Missouri 63110, United States

Veterans Affairs St. Louis Health Care System, 915 North Grand Boulevard, St. Louis, Missouri 63106, United States

Funding

Office of Naval Research · Division of Materials Research

References (65)

Show source-provided references

1. 10.1016/j.actbio.2018.04.03410.1016/j.actbio.2018.04.034 ↗

2. 10.1016/j.cobme.2021.10027110.1016/j.cobme.2021.100271 ↗

3. 10.1098/rsta.2011.048310.1098/rsta.2011.0483 ↗

4. 10.1002/pol.2021024910.1002/pol.20210249 ↗

5. 10.3390/bioengineering906025010.3390/bioengineering9060250 ↗

6. 10.1038/s41551-021-00769-y10.1038/s41551-021-00769-y ↗

7. 10.1002/adfm.20220228510.1002/adfm.202202285 ↗

8. 10.1038/s41467-021-23117-910.1038/s41467-021-23117-9 ↗

9. 10.1039/C7RA00861A10.1039/C7RA00861A ↗

10. 10.1371/journal.pone.015792010.1371/journal.pone.0157920 ↗

11. 10.1002/adhm.20150040610.1002/adhm.201500406 ↗

12. 10.1021/acs.biomac.7b0096910.1021/acs.biomac.7b00969 ↗

13. 10.1016/j.athoracsur.2008.12.07210.1016/j.athoracsur.2008.12.072 ↗

14. 10.1002/pat.446510.1002/pat.4465 ↗

15. 10.1016/j.sbi.2020.04.00710.1016/j.sbi.2020.04.007 ↗

16. 10.1002/chem.20210290210.1002/chem.202102902 ↗

17. 10.1002/adma.20190636010.1002/adma.201906360 ↗

18. 10.3390/molecules2813498810.3390/molecules28134988 ↗

19. 10.1016/j.mtbio.2022.10025610.1016/j.mtbio.2022.100256 ↗

20. 10.3390/ijms2407641610.3390/ijms24076416 ↗

21. 10.1021/acsami.1c1418210.1021/acsami.1c14182 ↗

22. 10.1021/acs.biomac.9b0021810.1021/acs.biomac.9b00218 ↗

23. 10.3390/ijms22191069810.3390/ijms221910698 ↗

24. 10.1021/acsami.8b1489010.1021/acsami.8b14890 ↗

25. 10.1038/s41598-020-76004-610.1038/s41598-020-76004-6 ↗

26. 10.1021/acs.biomac.8b0098010.1021/acs.biomac.8b00980 ↗

27. 10.1038/s41467-023-37563-010.1038/s41467-023-37563-0 ↗

28. 10.1186/1754-1611-5-1210.1186/1754-1611-5-12 ↗

29. 10.1007/s10853-008-2961-y10.1007/s10853-008-2961-y ↗

30. 10.1177/10.3.35510.1177/10.3.355 ↗

31. 10.1042/BSR2018141510.1042/BSR20181415 ↗

32. 10.1002/adma.20180495010.1002/adma.201804950 ↗

33. 10.1002/adhm.20190139610.1002/adhm.201901396 ↗

34. 10.1016/j.actbio.2018.09.02710.1016/j.actbio.2018.09.027 ↗

35. 10.1021/acsapm.1c0080710.1021/acsapm.1c00807 ↗

36. 10.1021/acsnano.1c0294410.1021/acsnano.1c02944 ↗

37. 10.1002/pol.2021091610.1002/pol.20210916 ↗

38. 10.1038/s41467-022-35437-510.1038/s41467-022-35437-5 ↗

39. 10.1021/acs.biomac.0c0106910.1021/acs.biomac.0c01069 ↗

40. 10.1016/j.compositesb.2021.10907110.1016/j.compositesb.2021.109071 ↗

41. 10.1016/j.cej.2021.13337210.1016/j.cej.2021.133372 ↗

42. 10.1016/S0142-9612(03)00257-610.1016/S0142-9612(03)00257-6 ↗

43. 10.1016/j.biomaterials.2017.01.03410.1016/j.biomaterials.2017.01.034 ↗

44. 10.1002/lary.2776910.1002/lary.27769 ↗

45. 10.1098/rsif.2018.083810.1098/rsif.2018.0838 ↗

46. 10.1016/j.actbio.2018.01.03210.1016/j.actbio.2018.01.032 ↗

47. 10.1038/s41467-018-03759-y10.1038/s41467-018-03759-y ↗

48. 10.1039/C7CC07215E10.1039/C7CC07215E ↗

49. 10.1039/c3tb00578j10.1039/c3tb00578j ↗

50. 10.1016/j.biomaterials.2015.03.00210.1016/j.biomaterials.2015.03.002 ↗

51. 10.1021/acsnano.7b0896910.1021/acsnano.7b08969 ↗

52. 10.1021/acsomega.8b0349210.1021/acsomega.8b03492 ↗

53. 10.1002/mren.20130011410.1002/mren.201300114 ↗

54. 10.1039/D0MH00361A10.1039/D0MH00361A ↗

55. Bunker D. L. J. · Muscles Ligaments Tendons J. · 2014 · 4 · 34310.32098/mltj.03.2014.13 ↗

56. 10.1007/s00167-015-3795-110.1007/s00167-015-3795-1 ↗

57. 10.1002/jor.2280610.1002/jor.22806 ↗

58. 10.1177/10711007000210070410.1177/107110070002100704 ↗

59. 10.1186/s13068-017-0936-410.1186/s13068-017-0936-4 ↗

60. 10.1038/s41467-021-25360-610.1038/s41467-021-25360-6 ↗

61. 10.1002/adma.20170484710.1002/adma.201704847 ↗

62. 10.1016/j.copbio.2017.10.01010.1016/j.copbio.2017.10.010 ↗

63. 10.1038/s41467-019-11285-810.1038/s41467-019-11285-8 ↗

64. 10.1038/s41467-022-31883-310.1038/s41467-022-31883-3 ↗

65. Westermark G. T. · 1999 · 309 · 3