Single-Step Synthesis of Highly Tunable Multifunctional Nanoliposomes for Synergistic Cancer Therapy

Cancer is still one of the major diseases that humans have not conquered yet. Nanotechnology has promoted the development of multifunctional nanoparticles, which integrate diagnostic and treatment abilities for tumor imaging and therapy. However, its preparation methods usually require complicated unit operations, which result in large batch-to-batch differences, poor reproducibility, high production costs, and difficulty in clinical transformation. Furthermore, precisely manufacturing nanoliposomes with different tunable features (e.g., size, surface charge, targeting ligands, and so forth) remains a challenge, limiting effective nanoliposome optimization for tumor therapy. Due to the accurate control of the synthesis process and continuous operation mode, microfluidic technology becomes an emerging approach for the manufacturing of nanoliposomes. However, there are few reports on the single-step preparation of complex nanoliposomes by precise tuning of the physical properties, while investigating the influence of anti-cancer efficiency. Herein, we have prepared multifunctional nanoliposomes with accurate tuning properties through a microfluidic device in a single step, with synergistic photodynamic and chemodynamic effects for targeted tumor therapy. The preparation method provides an effective way for the one-step preparation of multifunctional nanoparticles with controllable particle sizes and surface properties.