Ultrasensitive Electrochemiluminescence Biosensing Platform Based on Polymer Dots with Aggregation-Induced Emission for Dual-Biotoxin Assay

Multitarget assay has always been a hot topic in electrochemiluminescence (ECL) methods. Herein, a “on–off–on” ECL aptasensor was developed for the ultrasensitive and sequential detection of possible biological warfare agents, deoxynivalenol (DON) and abrin (ABR). As a luminophore, polymer dots (Pdots) with aggregation-induced emission exhibit high ECL efficiency in the aptasensor, i.e., the signal “on” state. The DON assays mainly depend on ECL quenching due to the efficient quenching effect between ferrocene-H2-ferrocene (Fc-H2-Fc) and Pdots, i.e., the signal “off” state. When the aptasensor is incubated with the oligonucleotide sequence S2 to replace Fc-H2-Fc, obvious ECL recovery occurs, i.e., the signal “on” state, which can be used to sequentially detect ABR. The limit of detection (LOD) for DON is 0.73 fg·mL –1 in the range of 5.0 to 50 ng·mL –1; and the LOD for ABR is ∼0.38 pg·mL –1 in the range of 1.25 pg·mL –1 to 1.25 μg·mL –1 . The as-designed ECL aptasensor exhibits good stability and reproducibility, high specificity, and favorable practicality. Therefore, this work provides a new approach for assays of DON and ABR in food safety and can be used as a model to design an ultrasensitive ECL biosensor for multitarget detection.