Physisorption of Affinity Ligands Facilitates Extracellular Vesicle Detection with Low Non-Specific Binding to Plasmonic Gold Substrates
Source abstract
Abstract
Plasmonic biosensors are increasingly being used for the analysis of extracellular vesicles (EVs) originating from disease areas. However, the high non-specific binding of EVs to a gold-sensing surface has been a critical problem and hindered the true translational potential. Here, we report that direct antibody immobilization on the plasmonic gold surface via physisorption shows excellent capture of cancer-derived EVs with ultralow non-specific binding even at very high concentrations. Contrary to commonly used methods that involve thiol-based linker attachment and an EDC/sulfo-NHS reaction, we show a higher specific capture rate and >50-fold lower non-specific on citrate-capped plain and nanopatterned gold surfaces. The method provides a simple, fast, and reproducible means to functionalize plasmonic gold surfaces with antibodies for robust EV biosensing.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Extracellular vesicles in disease · Advanced biosensing and bioanalysis techniques · Nanoplatforms for cancer theranostics
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, United States
Department of Radiology, Massachusetts General Hospital, Boston, Massachusetts 02114, United States
Cancer Center, Massachusetts General Hospital, Boston, Massachusetts 02114, United States
Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, United States
Funding
National Cancer Institute · Korea Health Industry Development Institute · National Research Foundation of Korea · Ministry of Trade, Industry and Energy · National Institute of General Medical Sciences
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