journal article·2023-06-07Open access

Layer-by-Layer Deposition of Low-Solid Nanochitin Emulgels Creates Porous Structures for High Cell Attachment and Proliferation

Ya Zhu, Esko Kankuri, Xue Zhang, Zhangmin Wan, Xin Wang, Siqi Huan, Long Bai, Shouxin Liu, Orlando J. Rojas

ACS Applied Materials & Interfaces· Volume 15· Issue 22

Abstract

Direct ink writing (DIW) is a customizable platform to engineer complex constructs from biobased colloids. However, the latter usually display strong interactions with water and lack interparticle connectivity, limiting one-step processing into hierarchically porous structures. We overcome such challenges by using low-solid emulgel inks stabilized by chitin nanofibrils (nanochitin, NCh). By using complementary characterization platforms, we reveal NCh structuring into spatially controlled three-dimensional (3D) materials that generate multiscale porosities defined by emulsion droplet size, ice templating, and DIW infill density. The extrusion variables, key in the development of surface and mechanical features of printed architectures, are comprehensively analyzed by using molecular dynamics and other simulation approaches. The obtained scaffolds are shown for their hierarchical porous structures, high areal density, and surface stiffness, which lead to excellent modulation of cell adhesion, proliferation, and differentiation, as tested with mouse dermal fibroblast expressing green fluorescent proteins.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Advanced Materials and Mechanics · Pickering emulsions and particle stabilization · Electrospun Nanofibers in Biomedical Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Biobased Colloids and Materials Group, Department of Bioproducts and Biosystems, Aalto University, Vuorimiehentie 1, P.O. Box 16300, 02150 Espoo, Finland

Department of Pharmacology, Faculty of Medicine, University of Helsinki, Viikinkaari 5E, P.O. Box 56, 00014 Helsinki, Finland

Bioproducts Institute, Department of Chemical & Biological Engineering, Department of Chemistry, and Department of Wood Science, The University of British Columbia, 2360 East Mall, Vancouver, British Columbia V6T 1Z3, Canada

Key Laboratory of Biobased Material Science and Technology (Ministry of Education), Northeast Forestry University, Harbin 150040, Heilongjiang, P. R. China

Funding

Canada Foundation for Innovation · Natural Science Foundation of Heilongjiang Province · Horizon 2020 Framework Programme · China Scholarship Council · Canada Excellence Research Chairs, Government of Canada

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