DataVXACS Applied Materials & InterfacesPaper
journal article·2023-02-22Open access

Superfluorinated Extracellular Vesicles for In Vivo Imaging by 19F-MRI

María Sancho-Albero, Nazeeha Ayaz, Victor Sebastian, Cristina Chirizzi, Miguel Encinas-Gimenez, Giulia Neri, Linda Chaabane, Lluís Luján, Pilar Martin-Duque, Pierangelo Metrangolo, Jesús Santamaría, Francesca Baldelli Bombelli

ACS Applied Materials & Interfaces· Volume 15· Issue 7
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Abstract

High Resolution Image Download MS PowerPoint Slide Extracellular vesicles (EVs) play a crucial role in cell-to-cell communication and have great potential as efficient delivery vectors. However, a better understanding of EV in vivo behavior is hampered by the limitations of current imaging tools. In addition, chemical labels present the risk of altering the EV membrane features and, thus, in vivo behavior. 19 F-MRI is a safe bioimaging technique providing selective images of exogenous probes. Here, we present the first example of fluorinated EVs containing PERFECTA, a branched molecule with 36 magnetically equivalent 19 F atoms. A PERFECTA emulsion is given to the cells, and PERFECTA-containing EVs are naturally produced. PERFECTA-EVs maintain the physicochemical features, morphology, and biological fingerprint as native EVs but exhibit an intense 19 F-NMR signal and excellent 19 F relaxation times. In vivo 19 F-MRI and tumor-targeting capabilities of stem cell-derived PERFECTA-EVs are also proved. We propose PERFECTA-EVs as promising biohybrids for imaging biodistribution and delivery of EVs throughout the body.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Extracellular vesicles in disease · MicroRNA in disease regulation · Nanoplatforms for cancer theranostics

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-Universidad de Zaragoza, 50009 Zaragoza, Spain

Department of Chemical Engineering and Environmental Technologies, University of Zaragoza, 50009 Zaragoza, Spain

Networking Research Center on Bioengineering Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain

Laboratory of Supramolecular and Bio-Nanomaterials (SupraBioNano Lab), Department of Chemistry, Materials and Chemical Engineering, “Giulio Natta”, Politecnico di Milano, 20131 Milan, Italy

Experimental Neurology (INSPE) and Experimental Imaging Center (CIS), Neuroscience Division, IRCCS Ospedale San Raffaele, 20132 Milan, Italy

Department of Animal Pathology, University of Zaragoza, 50009 Zaragoza, Spain

Instituto Universitario de Investigación Mixto Agroalimentario de Aragón (IA2), University of Zaragoza, 50009 Zaragoza, Spain

Instituto Aragonés de Ciencias de la Salud (IACS) /IIS Aragón, Zaragoza 5009, Spain

Fundación Araid, 50018 Zaragoza, Spain

Funding

Fundaci?n BBVA · Regione Lombardia · Fundaci?n Ram?n Areces · Centro de Investigaci?n Biom?dica en Red en Bioingenier?a, Biomateriales y Nanomedicina · European Regional Development Fund · Associazione Italiana per la Ricerca sul Cancro · Fondazione Cariplo · Ministero dell'Universit? e della Ricerca · Instituto de Salud Carlos III · H2020 European Research Council

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