DataVXACS Applied Materials & InterfacesPaper
journal article·2022-08-17

Quantitative and Noninvasive Detection of SAH-Related MiRNA in Cerebrospinal Fluids In Vivo Using SERS Sensors Based on Acupuncture-Based Technology

Jingyi Sun, Yanan Song, Mengyue Wang, Peng Zhao, Feng Gao, Junqi Li, Mingfeng Yang, Hui Yuan, Baoliang Sun, Ying Wang

ACS Applied Materials & Interfaces· Volume 14· Issue 32
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Source abstract

Abstract

Quantitative analysis of microRNAs (miRNAs) in a noninvasive manner is of vital importance for disease diagnosis and prognosis evaluation. However, conventional strategies for realizing accurate, simple, and sensitive detection of target molecules are still a challenge, especially for miRNAs due to their low abundance and susceptibility in the complex biological environment. Here, a novel surface-enhanced Raman scattering (SERS) strategy was established for quantitative detection and monitoring of miRNA-21-5p (miR-21-5p) in living cells and in vivo cerebrospinal fluid (CSF) by applying hairpin DNA (hpDNA)-conjugated gold nanostars (GNSs) SERS probes combined with acupuncture-based technology. This strategy enabled ultrasensitive exploration toward miR-21-5p in a wide range from 1 fM to 100 pM in cell lysates. Moreover, SERS analysis facilitated the detection and long-term monitoring for in vivo miR-21-5p noninvasively. This developed strategy promises to offer a powerful method for the analysis of multiple biomolecules in single cells and living bodies.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Gold and Silver Nanoparticles Synthesis and Applications · Extracellular vesicles in disease

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China

The Second Affiliated Hospital, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong 271000, China

Qingdao Medical College of Qingdao University, Qingdao, Shandong 266021, China

Department of Neurology, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, Haikou, Hainan 570102, China

Funding

Shandong First Medical University · Shandong Academy of Medical Sciences · Taishan Scholar Project of Shandong Province · National Natural Science Foundation of China · Natural Science Foundation of Shandong Province · Jinan Science and Technology Development Project · Shandong Provincial Medicine and Health Technology Development Plan · Tai'an Science and Technology Innovation Development Project

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