Source abstract
Abstract
Bacteria can colonize essentially any surface and form antibiotic resistant biofilms, which are multicellular structures embedded in an extracellular matrix secreted by the attached cells. To develop better biofilm control technologies, we recently demonstrated that mature biofilms can be effectively removed through on-demand shape recovery of a shape memory polymer (SMP) composed of tert-butyl acrylate (tBA). It was further demonstrated that such a dynamic substratum can sensitize the detached biofilm cells to antibiotics. However, this SMP can undergo shape change only once, limiting its application in long-term biofilm control. This motivated the present study, which aimed to prove the concept that biofilm can be effectively removed by repeated on-demand shape recovery. Reversible shape memory polymers (rSMPs) containing poly(ε-caprolactone) diisocyanatoethyl dimethacrylate (PCLDIMA) of varying molecular masses and butyl acrylate (BA) as a linker were synthesized by using benzoyl peroxide (BPO) as a thermal initiator. By comparison of several combinations of PCLDIMA of different molecular masses, a 2:1 weight ratio mixture of 2000 and 15000 g/mol PCLDIMA was the most promising because it had a shape transition (at 36.7 °C) close to body temperature. The synthesized rSMP demonstrated good reversible shape recovery and up to 94.3 ± 1.0% removal of 48 h Pseudomonas aeruginosa PAO1 biofilm cells after three consecutive shape recovery cycles. Additionally, the detached biofilm cells were found to be 5.0 ± 1.2 times more susceptible to 50 μg/mL tobramycin than the static control.
Source: openalex_inverted_index · Quality: baseline
Affiliations
Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, New York 13244, United States
Department of Chemistry, State University of New York - College of Environmental Science and Forestry, Syracuse, New York 13210, United States
The Michael M. Szwarc Polymer Research Institute, Syracuse, New York 13210, United States
Center for Devices and Radiological Health, Office of Science and Engineering Laboratories, Division of Biology, Chemistry, and Materials Science, United States Food and Drug Administration, Silver Spring, Maryland 20993, United States
Department of Civil and Environmental Engineering, Syracuse University, Syracuse, New York 13244, United States
Department of Biology, Syracuse University, Syracuse, New York 13244, United States
Funding
National Institute of Allergy and Infectious Diseases · National Institute of Biomedical Imaging and Bioengineering · Division of Materials Research