Dual-Peptide-Functionalized Nanofibrous Scaffolds Recruit Host Endothelial Progenitor Cells for Vasculogenesis to Repair Calvarial Defects

Li Li; Wanqian Liu; Yulan Zhao; Pingping Ma; Shenfang Zha; Peixin Chen; Hongwei Lu; Xiaorui Jiang; Shuang Wan; Jiangming Luo; Qijie Dai; Junxian Hu; Yohanes Kristo Sugiarto Utomo; Xinyun Han; Zhengwei Yang; Li Yang; Qingyi He

doi:10.1021/acsami.9b21434

journal article·2020-01-22

Dual-Peptide-Functionalized Nanofibrous Scaffolds Recruit Host Endothelial Progenitor Cells for Vasculogenesis to Repair Calvarial Defects

Li Li, Wanqian Liu, Yulan Zhao, Pingping Ma, Shenfang Zha, Peixin Chen, Hongwei Lu, Xiaorui Jiang, Shuang Wan, Jiangming Luo, Qijie Dai, Junxian Hu, Yohanes Kristo Sugiarto Utomo, Xinyun Han, Zhengwei Yang, Li Yang, Qingyi He

ACS Applied Materials & Interfaces· Volume 12· Issue 3

Publisher page

Source abstract

Abstract

Vasculogenesis (de novo formation of vessels) induced by endothelial progenitor cells (EPCs) is requisite for vascularized bone regeneration. However, there exist few available options for promoting vasculogenesis within artificial bone grafts except for exogenous EPC transplantation, which suffers from the source of EPC, safety, cost, and time concerns in clinical applications. This study aimed at endogenous EPC recruitment for vascularized bone regeneration by using a bioinspired EPC-induced graft. The EPC-induced graft was created by immobilizing two bioactive peptides, WKYMVm and YIGSR, on the surface of poly(ε-caprolactone) (PCL)/poliglecaprone (PGC) nanofibrous scaffolds via a polyglycolic acid (PGA)-binding peptide sequence. Remarkable immobilization efficacy of WKYMVm and YIGSR peptides and their sustained release (over 14 days) from scaffolds were observed. In vivo and in vitro studies showed robust recruitment of EPCs, which subsequently contributed to early vasculogenesis and ultimate bone regeneration. The dual-peptide-functionalized nanofibrous scaffolds proposed in this study provide a promising therapeutic strategy for vasculogenesis in bone defect repair.

Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials Biomaterials

OpenAlex topics: Bone Tissue Engineering Materials · Electrospun Nanofibers in Biomedical Applications · Protease and Inhibitor Mechanisms

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Orthopedic Department, Southwest Hospital, The First Hospital Affiliated to Army Medical University (Southwest Hospital), Chongqing 400038, P.R. China

Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, China

Orthopedic Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, P.R. China

Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, P.R. China

Center of Joint Surgery, Southwest Hospital, The First Hospital Affiliated to Army Medical University (Southwest Hospital), Chongqing 400038, P.R. China

Funding

Ministry of Education of the People's Republic of China · National Natural Science Foundation of China · Southwest Hospital · the Special Project of Enhancing the Scientific and Technological Innovation Ability of the Army Medical University · the Youth Training Program of Military Medical Science and Technology

References (69)

Show source-provided references

1. 10.1007/s00198-015-3154-610.1007/s00198-015-3154-6 ↗

2. 10.1016/j.bioactmat.2017.05.00710.1016/j.bioactmat.2017.05.007 ↗

3. 10.1002/adhm.20140076010.1002/adhm.201400760 ↗

4. 10.1016/j.biomaterials.2016.01.02410.1016/j.biomaterials.2016.01.024 ↗

5. 10.2353/ajpath.2006.06006410.2353/ajpath.2006.060064 ↗

6. 10.1111/jcpe.1232510.1111/jcpe.12325 ↗

7. 10.1126/science.275.5302.96410.1126/science.275.5302.964 ↗

8. 10.1152/ajpcell.00330.200310.1152/ajpcell.00330.2003 ↗

9. 10.1161/CIRCRESAHA.107.14893210.1161/CIRCRESAHA.107.148932 ↗

10. 10.1016/j.actbio.2011.07.00610.1016/j.actbio.2011.07.006 ↗

11. 10.1038/743410.1038/7434 ↗

12. 10.1016/j.bone.2008.01.00710.1016/j.bone.2008.01.007 ↗

13. 10.1002/jcp.2130910.1002/jcp.21309 ↗

14. 10.1002/jor.2213410.1002/jor.22134 ↗

15. 10.1002/stem.157810.1002/stem.1578 ↗

16. 10.1016/j.actbio.2015.07.03310.1016/j.actbio.2015.07.033 ↗

17. 10.1021/bm500385n10.1021/bm500385n ↗

18. 10.1039/C5NR05784A10.1039/C5NR05784A ↗

19. 10.1016/j.biomaterials.2004.02.01210.1016/j.biomaterials.2004.02.012 ↗

20. 10.1007/s10856-012-4736-x10.1007/s10856-012-4736-x ↗

21. 10.1186/1741-7015-9-6610.1186/1741-7015-9-66 ↗

22. 10.1016/j.nano.2016.05.01410.1016/j.nano.2016.05.014 ↗

23. 10.2147/IJN.S9744510.2147/IJN.S97445 ↗

24. 10.1016/j.biomaterials.2012.10.01610.1016/j.biomaterials.2012.10.016 ↗

25. 10.1016/j.biomaterials.2016.11.01810.1016/j.biomaterials.2016.11.018 ↗

26. 10.1016/j.apmt.2019.06.01510.1016/j.apmt.2019.06.015 ↗

27. 10.1021/acsami.7b1319910.1021/acsami.7b13199 ↗

28. 10.1002/adhm.20150034510.1002/adhm.201500345 ↗

29. 10.1007/s40204-015-0038-y10.1007/s40204-015-0038-y ↗

30. 10.1016/0142-9612(95)93577-Z10.1016/0142-9612(95)93577-Z ↗

31. 10.1016/j.biomaterials.2010.02.01710.1016/j.biomaterials.2010.02.017 ↗

32. 10.1021/acsami.5b1194910.1021/acsami.5b11949 ↗

33. 10.1016/j.msec.2018.08.00910.1016/j.msec.2018.08.009 ↗

34. 10.1021/acsami.8b2212310.1021/acsami.8b22123 ↗

35. 10.1016/j.biomaterials.2016.08.02310.1016/j.biomaterials.2016.08.023 ↗

36. 10.1111/j.1600-0501.2012.02458.x10.1111/j.1600-0501.2012.02458.x ↗

37. 10.1097/00001665-199807000-0000410.1097/00001665-199807000-00004 ↗

38. 10.1039/c8nr07329e10.1039/c8nr07329e ↗

39. 10.1002/adhm.20160043410.1002/adhm.201600434 ↗

40. 10.1021/acsami.7b1426710.1021/acsami.7b14267 ↗

41. 10.1016/j.jcis.2018.09.07110.1016/j.jcis.2018.09.071 ↗

42. 10.1021/la901242q10.1021/la901242q ↗

43. 10.1021/jp907731b10.1021/jp907731b ↗

44. 10.1021/am405101k10.1021/am405101k ↗

45. 10.1016/j.actbio.2019.02.02110.1016/j.actbio.2019.02.021 ↗

46. 10.1007/s10456-017-9571-810.1007/s10456-017-9571-8 ↗

47. 10.1002/bies.20130013310.1002/bies.201300133 ↗

48. 10.1016/j.bone.2008.05.00110.1016/j.bone.2008.05.001 ↗

49. 10.1089/ten.tea.2010.070810.1089/ten.tea.2010.0708 ↗

50. 10.1002/ccd.2292810.1002/ccd.22928 ↗

51. 10.1016/j.addr.2017.07.01110.1016/j.addr.2017.07.011 ↗

52. 10.1016/j.biomaterials.2013.08.06010.1016/j.biomaterials.2013.08.060 ↗

53. 10.1038/ncomms1037610.1038/ncomms10376 ↗

54. 10.1016/j.jcyt.2013.05.00410.1016/j.jcyt.2013.05.004 ↗

55. 10.1016/j.biomaterials.2017.05.00510.1016/j.biomaterials.2017.05.005 ↗

56. 10.1016/j.msec.2019.01.07510.1016/j.msec.2019.01.075 ↗

57. 10.1016/j.biopha.2018.06.11910.1016/j.biopha.2018.06.119 ↗

58. 10.3389/fbioe.2015.0007910.3389/fbioe.2015.00079 ↗

59. 10.1021/acsami.8b1168710.1021/acsami.8b11687 ↗

60. 10.1002/jor.2088610.1002/jor.20886 ↗

61. 10.1021/acsami.6b1053810.1021/acsami.6b10538 ↗

62. 10.1002/adfm.20160319710.1002/adfm.201603197 ↗

63. Risau W. · Development · 1988 · 102 · 47110.1242/dev.102.3.471 ↗

64. 10.1007/s10439-010-9986-510.1007/s10439-010-9986-5 ↗

65. 10.1021/am100970w10.1021/am100970w ↗

66. 10.1016/j.colsurfb.2016.06.03210.1016/j.colsurfb.2016.06.032 ↗

67. 10.1021/acsami.6b0868510.1021/acsami.6b08685 ↗

68. 10.1021/acsami.7b0332810.1021/acsami.7b03328 ↗

69. 10.1016/j.biomaterials.2013.03.05110.1016/j.biomaterials.2013.03.051 ↗