journal article·2023-04-26Open access

Empirical Optimization of Peptide Sequence and Nanoparticle Colloidal Stability: The Impact of Surface Ligands and Implications for Colorimetric Sensing

Zhicheng Jin, Justin Yeung, Jiajing Zhou, Maurice Retout, Wonjun Yim, Pavla Fajtová, Bryan Gosselin, Ivan Jabin, Gilles Bruylants, Hedi Mattoussi, Anthony J. O’Donoghue, Jesse V. Jokerst

ACS Applied Materials & Interfaces· Volume 15· Issue 16

Abstract

Surface ligands play a critical role in controlling and defining the properties of colloidal nanocrystals. These aspects have been exploited to design nanoparticle aggregation-based colorimetric sensors. Here, we coated 13-nm gold nanoparticles (AuNPs) with a large library of ligands ( e.g., from labile monodentate monomers to multicoordinating macromolecules) and evaluated their aggregation propensity in the presence of three peptides containing charged, thiolate, or aromatic amino acids. Our results show that AuNPs coated with the polyphenols and sulfonated phosphine ligands were good choices for electrostatic-based aggregation. AuNPs capped with citrate and labile-binding polymers worked well for dithiol-bridging and π–π stacking-induced aggregation. In the example of electrostatic-based assays, we stress that good sensing performance requires aggregating peptides of low charge valence paired with charged NPs with weak stability and vice versa . We then present a modular peptide containing versatile aggregating residues to agglomerate a variety of ligated AuNPs for colorimetric detection of the coronavirus main protease. Enzymatic cleavage liberates the peptide segment, which in turn triggers NP agglomeration and thus rapid color changes in <10 min. The protease detection limit is 2.5 nM.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Advanced Nanomaterials in Catalysis · Gold and Silver Nanoparticles Synthesis and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of NanoEngineering, University of California, San Diego, La Jolla, California 92093, United States

Department of Bioengineering, University of California San Diego, La Jolla, California 92093, United States

Materials Science and Engineering Program, University of California, San Diego, La Jolla, California 92093, United States

Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California 92093, United States

Laboratoire de Chimie Organique, Université libre de Bruxelles (ULB), avenue F. D. Roosevel 50, CP160/06, B-1050 Brussels, Belgium

Engineering of Molecular NanoSystems, Ecole Polytechnique de Bruxelles, Université libre de Bruxelles (ULB), avenue F. D. Roosevelt 50, CP165/64, B-1050 Brussels, Belgium

Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida 32306, United States

Department of Radiology, University of California, San Diego, La Jolla, California 92093, United States

Funding

Division of Chemistry · F?d?ration Wallonie-Bruxelles · National Institute of Dental and Craniofacial Research · National Institute of Allergy and Infectious Diseases · Air Force Office of Scientific Research · National Institute on Aging

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