Guiding Epilepsy Surgery with an LRP1-Targeted SPECT/SERRS Dual-Mode Imaging Probe

Zhi Li; Wanbing Sun; Wenjia Duan; Yiqing Jiang; Ming Chen; Guorong Lin; Qinyue Wang; Zhen Fan; Yusheng Tong; Luo Chen; Jianing Li; Guangli Cheng; Cong Wang; Cong Li; Liang Chen

doi:10.1021/acsami.2c02540

journal article·2023-01-11

Guiding Epilepsy Surgery with an LRP1-Targeted SPECT/SERRS Dual-Mode Imaging Probe

Zhi Li, Wanbing Sun, Wenjia Duan, Yiqing Jiang, Ming Chen, Guorong Lin, Qinyue Wang, Zhen Fan, Yusheng Tong, Luo Chen, Jianing Li, Guangli Cheng, Cong Wang, Cong Li, Liang Chen

ACS Applied Materials & Interfaces· Volume 15· Issue 1

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Source abstract

Abstract

Accurate identification of the resectable epileptic lesion is a precondition of operative intervention to drug-resistant epilepsy (DRE) patients. However, even when multiple diagnostic modalities are combined, epileptic foci cannot be accurately identified in ∼30% of DRE patients. Inflammation-associated low-density lipoprotein receptor-related protein-1 (LRP1) has been validated to be a surrogate target for imaging epileptic foci. Here, we reported an LRP1-targeted dual-mode probe that is capable of providing comprehensive epilepsy information preoperatively with SPECT imaging while intraoperatively delineating epileptic margins in a sensitive high-contrast manner with surface-enhanced resonance Raman scattering (SERRS) imaging. Notably, a novel and universal strategy for constructing self-assembled monolayer (SAM)-based Raman reporters was proposed for boosting the sensitivity, stability, reproducibility, and quantifiability of the SERRS signal. The probe showed high efficacy to penetrate the blood-brain barrier. SPECT imaging showed the probe could delineate the epileptic foci clearly with a high target-to-background ratio (4.11 ± 0.71, 2 h). Further, with the assistance of the probe, attenuated seizure frequency in the epileptic mouse models was achieved by using SPECT together with Raman images before and during operation, respectively. Overall, this work highlights a new strategy to develop a SPECT/SERRS dual-mode probe for comprehensive epilepsy surgery that can overcome the brain shift by the co-registration of preoperative SPECT and SERRS intraoperative images.

Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Epilepsy research and treatment · Nanoparticle-Based Drug Delivery

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, China

Department of Neurology, Shanghai Changhai Hospital, Second Military Medical University, Shanghai 200433, China

Department of Neurology and Research Center of Neurology in Second Affiliated Hospital and Key Laboratory of Medical Neurobiology of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310009, China

Key Laboratory of Smart Drug Delivery, Ministry of Education, State Key Laboratory of Medical Neurobiology, School of Pharmacy, Fudan University, Shanghai 201203, China

Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200040, China

Academy for Engineering and Technology, Fudan University, Shanghai 200433, China

Shanghai Center for Brain Science and Brain-Inspired Technology, Shanghai 201602, China

State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Shanghai 200032, China

National Center for Neurological Disorders, Shanghai 200040, China

Funding

Ministry of Science and Technology of the People's Republic of China · China Postdoctoral Science Foundation · Science and Technology Commission of Shanghai Municipality · National Natural Science Foundation of China · Shanghai Municipal Science and Technology Major Project · National Science Fund for Distinguished Young Scholars · National Postdoctoral Program for Innovative Talents

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