Toward Spontaneous Neuronal Differentiation of SH-SY5Y Cells Using Novel Three-Dimensional Electropolymerized Conductive Scaffolds
Source abstract
Abstract
Neuroblastoma-derived SH-SY5Y cells have become an excellent model for nervous system regeneration to treat neurodegenerative disorders. Many approaches achieved a mature population of derived neurons in in vitro plates. However, the importance of the third dimension in tissue regeneration has become indispensable to achieve a potential implant to replace the damaged tissue. Therefore, we have prepared porous 3D structures composed uniquely of carbon nanotubes (CNT) and poly(3,4-ethylenedioxythiophene) (PEDOT) that show great potential in the tridimensional differentiation of SH-SY5Y cells into mature neurons. The scaffolds have been manufactured through electropolymerization by applying 1.2 V in a three-electrode cell using a template of sucrose/CNT as a working electrode. By this method, PEDOT/CNT 3D scaffolds were obtained with homogeneous porosities and high conductivity. In vitro analyses showed that an excellent biocompatibility of the scaffold and the presence of high amount of β-tubulin class III and MAP-II target proteins that mainly expresses in neurons, suggesting the differentiation into neuronal cells already after a week of incubation.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Neuroscience and Neural Engineering · Conducting polymers and applications · Graphene and Nanomaterials Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 182, 20014 Donostia San Sebastián, Spain
POLYMAT University of the Basque Country UPV/EHU, Avenida de Tolosa 72, 20018 Donostia-San Sebastián, Spain
Cardiovascular Institute, UC Denver Anschutz Medical Campus, School of Medicine, 12700 E. 19th Avenue, Bldg. P15, Aurora, Colorado 80045, United States
Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain
Department of Chemical and Pharmaceutical Sciences, INSTM, University of Trieste, Via L. Giorgieri 1, 34127 Trieste, Italy
Funding
AXA Research Fund · Ministerio de Econom?a y Competitividad · H2020 Marie Sklodowska-Curie Actions · Eusko Jaurlaritza · Universit? degli Studi di Trieste · Diputaci?n Foral de Gipuzkoa · Agencia Estatal de Investigaci?n, Ministerio de Ciencia, Innovaci?n y Universidades
References (57)
Show source-provided references
1. 10.1038/s41598-018-33710-610.1038/s41598-018-33710-6 ↗
2. 10.12659/MSM.91551810.12659/MSM.915518 ↗
3. 10.1016/j.neulet.2015.10.04710.1016/j.neulet.2015.10.047 ↗
4. 10.1186/s13024-017-0149-010.1186/s13024-017-0149-0 ↗
5. 10.1046/j.1471-4159.2000.0750991.x10.1046/j.1471-4159.2000.0750991.x ↗
6. 10.3233/JAD-2010-09136310.3233/JAD-2010-091363 ↗
7. 10.1016/j.tiv.2015.11.00410.1016/j.tiv.2015.11.004 ↗
8. 10.1002/smll.20110100410.1002/smll.201101004 ↗
9. 10.1166/jbn.2014.199510.1166/jbn.2014.1995 ↗
10. 10.1021/acsami.8b1256210.1021/acsami.8b12562 ↗
11. 10.1002/adhm.20160024510.1002/adhm.201600245 ↗
12. 10.1039/c3tb20336k10.1039/c3tb20336k ↗
13. 10.1385/JMN:14:3:17510.1385/JMN:14:3:175 ↗
14. 10.1016/j.addr.2013.07.00210.1016/j.addr.2013.07.002 ↗
15. 10.1002/adfm.20170055010.1002/adfm.201700550 ↗
16. 10.1073/Proc.Natl.Acad.SocU.S.A..200570811710.1073/Proc.Natl.Acad.SocU.S.A..2005708117 ↗
17. 10.1038/nrn182710.1038/nrn1827 ↗
18. 10.1016/j.carbon.2018.11.02610.1016/j.carbon.2018.11.026 ↗
19. 10.1038/nnano.2008.37410.1038/nnano.2008.374 ↗
20. 10.1523/JNEUROSCI.1332-11.201110.1523/JNEUROSCI.1332-11.2011 ↗
21. 10.1523/JNEUROSCI.1051-07.200710.1523/JNEUROSCI.1051-07.2007 ↗
22. Fabbro A. · 2011 · 194 · 24110.1016/B978-0-444-53815-4.00003-0 ↗
23. 10.1021/acsami.7b1325710.1021/acsami.7b13257 ↗
24. 10.1016/j.nano.2009.02.00610.1016/j.nano.2009.02.006 ↗
25. Nurulhuda I. · 2014
26. 10.1039/D0RA03383A10.1039/D0RA03383A ↗
27. 10.1126/sciadv.160008710.1126/sciadv.1600087 ↗
28. 10.1002/adbi.20190023810.1002/adbi.201900238 ↗
29. 10.1152/physiol.00036.201610.1152/physiol.00036.2016 ↗
30. 10.1016/j.snb.2009.04.06210.1016/j.snb.2009.04.062 ↗
31. 10.3390/polym805018510.3390/polym8050185 ↗
32. 10.1039/C6TB00852F10.1039/C6TB00852F ↗
33. 10.1016/j.tsf.2012.07.01910.1016/j.tsf.2012.07.019 ↗
34. 10.1021/acsami.7b0229610.1021/acsami.7b02296 ↗
35. Ali-Boucetta, H.; Al-Jamal, K. T.; Kostarelos, K., Cytotoxic Assessment of Carbon Nanotube Interaction with Cell Cultures. In Biomedical Nanotechnology: Methods and Protocols, Hurst, S. J., Ed. Humana Press: Totowa, NJ, 2011; pp 299–312.10.1007/978-1-61779-052-2_19 ↗
36. 10.1016/j.bios.2014.09.00410.1016/j.bios.2014.09.004 ↗
37. 10.1016/j.jconrel.2016.10.02010.1016/j.jconrel.2016.10.020 ↗
38. 10.1088/1748-6041/4/4/04500910.1088/1748-6041/4/4/045009 ↗
39. 10.1016/j.actbio.2004.09.00610.1016/j.actbio.2004.09.006 ↗
40. 10.1002/celc.20160043910.1002/celc.201600439 ↗
41. 10.1039/C7TB00608J10.1039/C7TB00608J ↗
42. 10.1021/acs.biomac.8b0138210.1021/acs.biomac.8b01382 ↗
43. 10.1021/acsbiomaterials.9b0131610.1021/acsbiomaterials.9b01316 ↗
44. 10.1021/acsami.5b0733210.1021/acsami.5b07332 ↗
45. 10.1039/C9TA03153G10.1039/C9TA03153G ↗
46. 10.1016/j.electacta.2014.10.11410.1016/j.electacta.2014.10.114 ↗
47. 10.1016/j.cclet.2013.12.02010.1016/j.cclet.2013.12.020 ↗
48. 10.1002/marc.20080055010.1002/marc.200800550 ↗
49. 10.1016/j.synthmet.2010.01.03310.1016/j.synthmet.2010.01.033 ↗
50. 10.1002/app.3425710.1002/app.34257 ↗
51. 10.1016/j.electacta.2011.01.06310.1016/j.electacta.2011.01.063 ↗
52. 10.3390/polym905015710.3390/polym9050157 ↗
53. 10.1039/C9NR00765B10.1039/C9NR00765B ↗
54. 10.1186/1556-276X-9-55710.1186/1556-276X-9-557 ↗
55. 10.1016/j.jpowsour.2017.09.08410.1016/j.jpowsour.2017.09.084 ↗
56. 10.1523/JNEUROSCI.06-03-00714.198610.1523/JNEUROSCI.06-03-00714.1986 ↗
57. 10.1007/s12031-019-01291-210.1007/s12031-019-01291-2 ↗