Imidazolium-Modified Bispyrene-Based Fluorescent Aggregates for Discrimination of Multiple Anions in Aqueous Solution

A great number of anions exist in biological systems and natural environment, and are highly relevant to human health and environment quality. It is necessary to develop simple and effective sensors to differentiate and identify those similar or different anions. Here, an imidazolium-modified bispyrene-based fluorescent amphiphilic probe DPyDIM was synthesized and its aggregates were applied to detect and discriminate various anions. The fluorescent aggregates exhibit ratiometric responses to different types of anions. Moreover, the ratiometric responses to different types of anions are featured with multiple-wavelength cross-reactivity. The collection of fluorescence variation at four typical wavelengths can generate distinct recognition patterns to specific anions. The heat map and principal component analysis results verify that this single fluorescent sensor system can effectively and sensitively identify 16 kinds of anions that belong to phosphorus-containing, sulfur-containing anions, and anionic surfactants. The cross-reactive sensing of the amphiphilic fluorescent aggregates was attributed to the different influences on the aggregation behaviors of the probes by different anions. The present work provides a promising strategy for effective detection and discrimination of multiple anions by employing dynamic fluorescent aggregates as a sensing platform.