Source abstract
Abstract
Poly(dimethylsiloxane) (PDMS) is a commonly used polymer in organ-on-a-chip devices and microphysiological systems. However, due to its hydrophobicity and permeability, it absorbs drug compounds, preventing accurate drug screening applications. Here, we developed an effective and facile method to prevent the absorption of drugs by utilizing a PDMS-PEG block copolymer additive and drug pretreatment. First, we incorporated a PDMS-PEG block copolymer into PDMS to address its inherent hydrophobicity. Next, we addressed the permeability of PDMS by eliminating the concentration gradient via pretreatment of the PDMS with the drug prior to experimentally testing drug absorption. The combined use of a PDMS-PEG block copolymer with drug pretreatment resulted in a mean reduction of drug absorption by 91.6% in the optimal condition. Finally, we demonstrated that the proposed method can be applied to prevent drug absorption in a PDMS-based cardiac microphysiological system, enabling more accurate drug studies.
Source: openalex_inverted_index · Quality: baseline
Affiliations
Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, United States
Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington 98195, United States
Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington 98195, United States
Department of Material Science and Engineering, University of Washington, Seattle, Washington 98195, United States
Department of Pathology and Laboratory Medicine, Brown University, Providence, Rhode Island 02912, United States
Department of Mechanical Engineering, University of Washington, Seattle, Washington 98195, United States
Department of Bioengineering, University of Washington, Seattle, Washington 98195, United States
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, United States
Funding
National Center for Advancing Translational Sciences · National Heart, Lung, and Blood Institute · American Heart Association · National Institutes of Health
References (40)
Show source-provided references
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