journal article·2021-09-22

Aqueous Protein–Polymer Bioconjugation via Photoinduced RAFT Polymerization Using High Loading Heterogeneous Catalyst

Ya Huang, Xue Li, Yu Chi Zhang, Zhiwei Shi, Lun Zeng, Jianbo Xie, Yucong Du, Dong Lu, Zhigang Hu, Tao Cai, Zhengtang Luo

ACS Applied Materials & Interfaces· Volume 13· Issue 37
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Abstract

Light-driven polymerization, such as photoinduced electron/energy transfer-reversible addition-fragmentation chain transfer (PET-RAFT) polymerization, enables biological benign conditions and versatile functional polymer structure design, which is readily used in protein-polymer bioconjugates. However, conventional metalloporphyrinic homogeneous catalysts for PET-RAFT polymerization suffer from limited aqueous solubility and tedious purification. Here we demonstrate the design of PET-RAFT photocatalyst from the reticular assembled Zr-porphyrinic metal-organic frameworks (MOFs), along with a biomacromolecule-based chain transfer agent, as efficient bioconjugation tools in water. Our methodology offers manufacturing advantages on bioconjugates under mild conditions such that MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) and cytotoxicity assays have shown the preservation of the protein integrity, bioactivity, and high cell viability after PET-RAFT polymerization. We find that the fast kinetics are benefiting from the ultrahigh loading of metalloporphyrins in MOF-525-Zn. This heterogeneous catalyst also allows us to maintain living characteristics to incorporate myriads of monomers into block copolymers. Other advantages like easy postreaction purification, reusability, and high oxygen tolerance even in an open system are demonstrated. This study provides a tool of highly efficient heterogeneous photocatalysts for polymer-protein bioconjugation in aqueous media and paves the road for biological applications.
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Research context

OpenAlex topics: Advanced Polymer Synthesis and Characterization · Photopolymerization techniques and applications · Click Chemistry and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Key Laboratory of Biomedical Polymers of Ministry of Education, College of Chemistry and Molecular Science, Wuhan University, Wuhan, Hubei 430072, P. R. China

Department of Chemical and Biological Engineering, Guangdong-Hong Kong-Macao Joint Laboratory for Intelligent Micro-Nano Optoelectronic Technology, William Mong Institute of Nano Science and Technology, and Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, P. R. China

Guangzhou Baiyun Medical Adhesive Company Ltd., Guangzhou, Guangdong 510405, P. R. China

Guangzhou HKUST Fok Ying Tung Research Institute, Guangzhou, Guangdong 511458, P. R. China

Silver Age Engineering Plastics (Dongguan) Company Ltd., Dongguan, Guangdong 523187, P. R. China

Funding

Guangdong Science and Technology Department · Zhongshan Science and Technology Bureau · Research Grants Council, University Grants Committee · Guangzhou Municipal Science and Technology Bureau · National Natural Science Foundation of China · Research Fund of Guangdong-Hong Kong-Macao Joint Laboratory for Intelligent Micro-Nano Optoelectronic Technology · IER foundation · NSFC-RGC Joint Research Scheme

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