journal article·2021-08-11

Co-assembly of Peptides and Carbon Nanodots: Sensitive Analysis of Transglutaminase 2

Yiwei Han, Yichen Zhang, Shuai Wu, Mohammed Jalalah, Saeed A. Alsareii, Yongmei Yin, Farid A. Harraz, Genxi Li

ACS Applied Materials & Interfaces· Volume 13· Issue 31
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Abstract

The structures assembled by peptides have attracted great attention due to their unique physicochemical properties. Moreover, the co-assembly of peptides with additional components can endow the structures with extended functions. In this work, we have explored the co-assembly of peptides and carbon nanodots (CNDs) by taking advantage of their non-covalent binding; thus, the obtained structure may show both the recognition capability of peptides and the catalytic activity of CNDs. Therefore, we have further used the assembled structure for the sensitive analysis of transglutaminase 2 with a low detection limit of 0.25 pg/mL. By simply replacing the peptide sequences or the nanomaterials, the strategy proposed in this work can be developed as a universal model to build the co-assemblies of peptides and nanomaterials, thus leading to their broader applications in biological and biomedical research.
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Research context

OpenAlex topics: Polymer Surface Interaction Studies · Supramolecular Self-Assembly in Materials · Proteins in Food Systems

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, P. R. China

Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, P. R. China

Promising Centre for Sensors and Electronic Devices (PCSED), Advanced Materials and Nano-Research Centre, Najran University, P.O. Box: 1988, Najran 11001, Saudi Arabia

Department of Electrical Engineering, Faculty of Engineering, Najran University, Najran 11001, Saudi Arabia

Department of Surgery, College of Medicine, Najran University, Najran 11001, Saudi Arabia

Nanomaterials and Nanotechnology Department, Central Metallurgical Research and Development Institute (CMRDI), P.O. Box 87 Helwan, Cairo 11421, Egypt

Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, P. R. China

Funding

Central University Basic Research Fund of China · National Natural Science Foundation of China · Deputyship for Research & Innovation, Ministry of Education in Saudi Arabia

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