DataVXACS Applied Materials & InterfacesPaper
journal article·2017-11-22

Eumelanin Coated PLA Electrospun Micro Fibers as Bioinspired Cradle for SH-SY5Y Neuroblastoma Cells Growth and Maturation

Ines Fasolino, Irene Bonadies, Luigi Ambrosio, Maria Grazia Raucci, Cosimo Carfagna, Federica Maria Caso, Francesca Cimino, Alessandro Pezzella

ACS Applied Materials & Interfaces· Volume 9· Issue 46
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Source abstract

Abstract

Within the framework of neurodegenerative disorder therapies, the fabrication of 3D eumelanin architectures represents a novel strategy to realize tissue-engineering scaffolds for neuronal cell growth and control by providing both mechanical support and biological signals. Here, an appropriate procedure combining electrospinning, spin coating and solid-state polymerization process is established to realize the scaffolds. For biological analysis, a human derived cell line SH-SY5Y from neuroblastoma is used. Cell maturation on eumelanin microfibers, random and aligned, is evaluated by using confocal analysis and specific markers of differentiating neurons (βIII tubulin and GAP-43 expression). Cell morphology is tested by SEM analysis and immunofluorescence techniques. As results, eumelanin coated microfibers prove capable to support biological response in terms of cell survival, adhesion and spreading and to promote cell differentiation toward a more mature neuronal phenotype as confirmed by GAP-43 expression over the culture.
Source: openalex_inverted_index · Quality: baseline

Research context

Polymers

OpenAlex topics: Silk-based biomaterials and applications · Electrospun Nanofibers in Biomedical Applications · Nerve injury and regeneration

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Polymers, Composites and Biomaterials (IPCB) − CNR, Institute of Polymers, Viale J.F. Kennedy 54, Mostra D’Oltremare Pad 20, 80125 Naples, Italy

Institute of Polymers, Composites and Biomaterials (IPCB) − CNR, Via Campi Flegrei 34, 80078 Pozzuoli (Na), Italy

Department of Chemical, Materials and Production Engineering (DICMAPI), University of Naples Federico II, P. le Tecchio 80, 80125 Napoli, Italy

Nanofaber Spin-off at Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Casaccia Research Centre, Via Anguillarese 301, 00123 Rome, Italy

Department of Chemical Sciences, University of Naples Federico II, Via Cintia 4, 80126 Naples, Italy

National Interuniversity Consortium of Materials Science and Technology (INSTM), Via G. Giusti, 9, 50121 Florence, Italy

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