NiCoMnO4: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition
Source abstract
Abstract
A bifunctional affinity probe NiCoMnO4 was designed and prepared with controllable morphology and size using facile methods. It was observed that the probe could be applied in His-tagged proteins purification and phosphopeptides enrichment simply through the buffer modulation. NiCoMnO4 particles showed satisfactory cycling performance for His-tagged proteins purification and broad pH-tolerance of loading buffer for phosphopeptides affinity. Therefore, a high-throughput, cost-effective, and efficient protein/peptide purification method was developed within 10 min based on the novel bifunctional affinity probe.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Glycosylation and Glycoproteins Research · Monoclonal and Polyclonal Antibodies Research · Advanced biosensing and bioanalysis techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Beijing Nuclear Magnetic Resonance Center, College of Life Science, Peking University, Beijing 100871, China
Funding
National Natural Science Foundation of China
References (47)
Show source-provided references
1. 10.1038/nature1539110.1038/nature15391 ↗
2. 10.1038/nature1619110.1038/nature16191 ↗
3. 10.1016/S0968-0004(00)89045-310.1016/S0968-0004(00)89045-3 ↗
4. 10.1074/jbc.M114.54941010.1074/jbc.M114.549410 ↗
5. 10.1111/mmi.1262810.1111/mmi.12628 ↗
6. 10.1021/ja063177n10.1021/ja063177n ↗
7. 10.1016/j.msec.2013.01.01110.1016/j.msec.2013.01.011 ↗
8. 10.1007/s11051-014-2358-610.1007/s11051-014-2358-6 ↗
9. 10.1039/c3tb20573h10.1039/c3tb20573h ↗
10. 10.1039/B715796G10.1039/B715796G ↗
11. 10.1021/am505041a10.1021/am505041a ↗
12. 10.1007/s10854-012-0776-810.1007/s10854-012-0776-8 ↗
13. 10.1039/C5CC00318K10.1039/C5CC00318K ↗
14. 10.1038/nrmicro340210.1038/nrmicro3402 ↗
15. 10.1038/ncomms596110.1038/ncomms5961 ↗
16. 10.1038/nrm399910.1038/nrm3999 ↗
17. 10.1038/cdd.2014.12810.1038/cdd.2014.128 ↗
18. 10.1038/ncomms318910.1038/ncomms3189 ↗
19. 10.1038/labinvest.2014.13110.1038/labinvest.2014.131 ↗
20. 10.1038/cr.2015.3010.1038/cr.2015.30 ↗
21. 10.1038/nrm397910.1038/nrm3979 ↗
22. 10.1021/ja308453m10.1021/ja308453m ↗
23. 10.1038/nmeth.270310.1038/nmeth.2703 ↗
24. 10.1039/c3an36853j10.1039/c3an36853j ↗
25. 10.1021/ac400198n10.1021/ac400198n ↗
26. 10.1038/ncomms266210.1038/ncomms2662 ↗
27. 10.1039/c3tb20127a10.1039/c3tb20127a ↗
28. 10.1021/am502530c10.1021/am502530c ↗
29. 10.1039/c3cc38909j10.1039/c3cc38909j ↗
30. 10.1007/s10562-009-9931-010.1007/s10562-009-9931-0 ↗
31. 10.1016/j.molcata.2013.11.01710.1016/j.molcata.2013.11.017 ↗
32. 10.1039/C5RA16626H10.1039/C5RA16626H ↗
33. 10.1016/j.jallcom.2009.11.18910.1016/j.jallcom.2009.11.189 ↗
34. 10.1039/C3DT52084F10.1039/C3DT52084F ↗
35. 10.1002/adma.20090185810.1002/adma.200901858 ↗
36. 10.1016/j.jcis.2013.11.00410.1016/j.jcis.2013.11.004 ↗
37. 10.1021/ac401668e10.1021/ac401668e ↗
38. 10.1002/adfm.20120136410.1002/adfm.201201364 ↗
39. 10.1002/anie.20121031710.1002/anie.201210317 ↗
40. 10.1039/C4CC04090B10.1039/C4CC04090B ↗
41. 10.1039/c3tb21617a10.1039/c3tb21617a ↗
42. 10.1039/c2cc31641b10.1039/c2cc31641b ↗
43. 10.1021/ac801974f10.1021/ac801974f ↗
44. 10.1021/ac301119r10.1021/ac301119r ↗
45. 10.1016/j.jchromb.2012.06.03310.1016/j.jchromb.2012.06.033 ↗
46. 10.1002/jssc.20120082110.1002/jssc.201200821 ↗
47. 10.1016/j.jchromb.2012.12.01310.1016/j.jchromb.2012.12.013 ↗