DataVXACS Applied Materials & InterfacesPaper
journal article·2020-08-26

Aptamer-Directed Protein-Specific Multiple Modifications of Membrane Glycoproteins on Living Cells

Xigao Chen, Liping Qiu, Ren Cai, Cheng Cui, Long Li, Jian-hui Jiang, Weihong Tan

ACS Applied Materials & Interfaces· Volume 12· Issue 34
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Source abstract

Abstract

Understanding how a cell membrane protein functions on living cells remains a challenge for cell biology. Specific placement of functional molecules on specific proteins in their native environment would allow comprehensive study of proteins’ dynamic functions. Existing methods cannot facilely achieve multiple modifications on specific membrane proteins. In this report, we describe an aptamer-induced, protein-specific bio-orthogonal modification technology for precise nongenetic immobilization of multiple small functional molecules on target membrane glycoproteins by combining metabolic technology and aptamer targeting. In brief, DNA probes were designed by modifying aptamers, which bind to target proteins on the surfaces of living cells pretreated with N-azidoacetylmannosamine-tetraacylated (Ac4ManNAz). The cyclooctynes tagged of DNA probes will approach the azide groups to trigger the bio-orthogonal reactions. After UV irradiation and hybridization with cDNA (complementary DNA), the aptamers can be removed, and the process can be repeated to achieve multiple modifications for multicolor imaging and cell surface nanoengineering on specific proteins.
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Research context

Nanomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Click Chemistry and Applications · RNA Interference and Gene Delivery

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Center for Research at Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611-7200, United States

Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China

The Cancer Hospital of the University of Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China

Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China

Funding

National Cancer Institute · Ministry of Science and Technology of the People's Republic of China · National Natural Science Foundation of China · National Institute of General Medical Sciences

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