journal article·2020-09-09

Mechanism of Nanoformulated Graphene Oxide-Mediated Human Neutrophil Activation

Yu-Jen Lu, Yi-Hsuan Wang, Rama Shanker Sahu, Jyh-Ping Chen, Banendu Sunder Dash, Pei-Jen Chung, Hung-Wei Yang, Er-Yuan Chuang, Tsong-Long Hwang

ACS Applied Materials & Interfaces· Volume 12· Issue 36
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Abstract

Understanding the molecular mechanisms of graphene oxide (GO)-based biomaterials is important for logical biomedical applications. Previous studies have revealed biointeractions between GO and immune effector cells, but the effects on neutrophils, crucial cells in the immune system, have not been thoroughly discussed. In this study, GO nanoformulations were synthesized with different functional groups, including GO, GO-carboxylated (GO-COOH), and PEGylated GO (GO-PEG), with different surface features, which were elucidated using imaging methods and surface-sensitive quantitative spectroscopic techniques, including atomic force microscopy (AFM), transmission electron microscopy (TEM), and X-ray photoemission spectroscopy (XPS). The GO-based nanoformulations elicited reactive oxygen species (ROS) generation and neutrophil extracellular trap (NET) formation in human neutrophils. Nanoformulated GO stimulates NET development via the formation of ROS. An endocytosis study revealed that nanoformulated GO facilitated internalization by neutrophils via macropinocytosis and actin-dependent phagocytosis. Importantly, calcium mobilization and phosphorylation proteins such as mitogen-activated protein kinases (extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38) and AKT were involved in the activation of neutrophils. These findings offer the first verification that nanoformulated GO exhibits direct effects on human neutrophils.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Neutrophil, Myeloperoxidase and Oxidative Mechanisms · Graphene and Nanomaterials Applications · Advanced Nanomaterials in Catalysis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Neurosurgery, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan

Graduate Institute of Natural Products, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan

Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan

Department of Chemical and Materials Engineering, Chang Gung University, Taoyuan 33302, Taiwan

Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan

Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, International Ph.D. Program of Biomedical Engineering, Taipei Medical University, Taipei 110, Taiwan

Cell Physiology and Molecular Image Research Center, Taipei Medical University—Wan Fang Hospital, Taipei 116, Taiwan

Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety, Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan

Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 33302, Taiwan

Department of Chemical Engineering, Ming-Chi University of Technology, New Taipei City 243, Taiwan

Funding

Chang Gung Memorial Hospital, Linkou · Ministry of Science and Technology, Taiwan

References (48)

Show source-provided references
1. 10.1210/en.2009-108210.1210/en.2009-1082 ↗
2. 10.3389/fimmu.2017.0047110.3389/fimmu.2017.00471 ↗
3. 10.1039/C5TB00180C10.1039/C5TB00180C ↗
4. 10.1016/j.mattod.2013.09.00410.1016/j.mattod.2013.09.004 ↗
5. 10.1038/am.2017.17110.1038/am.2017.171 ↗
6. 10.1039/c3nr05559k10.1039/c3nr05559k ↗
7. 10.1016/j.micinf.2003.09.00810.1016/j.micinf.2003.09.008 ↗
8. 10.1146/annurev.immunol.23.021704.11565310.1146/annurev.immunol.23.021704.115653 ↗
9. 10.4049/jimmunol.120171910.4049/jimmunol.1201719 ↗
10. 10.1038/nnano.2010.4410.1038/nnano.2010.44 ↗
11. 10.1002/smll.20150003810.1002/smll.201500038 ↗
12. 10.1016/j.proeng.2017.04.11810.1016/j.proeng.2017.04.118 ↗
13. 10.2147/IJN.S6676810.2147/IJN.S66768 ↗
14. 10.1002/ppsc.20140009910.1002/ppsc.201400099 ↗
15. 10.1186/1477-3155-12-510.1186/1477-3155-12-5 ↗
16. 10.1186/s40580-016-0064-z10.1186/s40580-016-0064-z ↗
17. 10.1016/j.jconrel.2014.06.03810.1016/j.jconrel.2014.06.038 ↗
18. 10.1016/j.msec.2016.09.01610.1016/j.msec.2016.09.016 ↗
19. 10.1155/2015/81703810.1155/2015/817038 ↗
20. 10.1007/s00592-008-0040-010.1007/s00592-008-0040-0 ↗
21. 10.1038/srep1820410.1038/srep18204 ↗
22. 10.1002/adhm.20150060610.1002/adhm.201500606 ↗
23. 10.1038/ni.298710.1038/ni.2987 ↗
24. 10.1073/pnas.141405511210.1073/pnas.1414055112 ↗
25. 10.1016/S0167-4889(98)00081-010.1016/S0167-4889(98)00081-0 ↗
26. 10.1074/jbc.272.33.2033210.1074/jbc.272.33.20332 ↗
27. 10.4161/cl.2396710.4161/cl.23967 ↗
28. 10.1021/mp300238w10.1021/mp300238w ↗
29. Ivanov A. I. · 2008 · 1510.1007/978-1-59745-178-9_2 ↗
30. 10.2174/187152301131202000810.2174/1871523011312020008 ↗
31. 10.26355/eurrev_201810_1617810.26355/eurrev_201810_16178 ↗
32. 10.3389/fimmu.2012.0038010.3389/fimmu.2012.00380 ↗
33. 10.3389/fimmu.2018.0028810.3389/fimmu.2018.00288 ↗
34. 10.1073/pnas.160223011310.1073/pnas.1602230113 ↗
35. 10.1038/s41418-018-0261-x10.1038/s41418-018-0261-x ↗
36. 10.1155/2016/460471310.1155/2016/4604713 ↗
37. 10.3389/fimmu.2013.0004510.3389/fimmu.2013.00045 ↗
38. 10.4049/jimmunol.175.6.409110.4049/jimmunol.175.6.4091 ↗
39. 10.7554/eLife.2443710.7554/eLife.24437 ↗
40. 10.1158/1078-0432.CCR-13-168310.1158/1078-0432.CCR-13-1683 ↗
41. 10.1371/journal.ppat.100419710.1371/journal.ppat.1004197 ↗
42. 10.1073/pnas.171103711510.1073/pnas.1711037115 ↗
43. 10.4049/jimmunol.177.6.403710.4049/jimmunol.177.6.4037 ↗
44. 10.1128/MCB.19.7.479810.1128/MCB.19.7.4798 ↗
45. Zu Y. L. · J. Immunol. · 1998 · 160 · 198210.4049/jimmunol.160.4.1982 ↗
46. 10.1038/sj.bjp.070456110.1038/sj.bjp.0704561 ↗
47. 10.4049/jimmunol.167.10.594010.4049/jimmunol.167.10.5940 ↗
48. 10.1016/j.bbamcr.2018.08.01410.1016/j.bbamcr.2018.08.014 ↗