Bactericidal Effect of 5-Mercapto-2-nitrobenzoic Acid-Coated Silver Nanoclusters against Multidrug-Resistant Neisseria gonorrhoeae
Source abstract
Abstract
Neisseria gonorrhoeae is among the most multidrug-resistant bacteria in circulation today, and new treatments are urgently needed. In this work, we demonstrate the ability of 5-mercapto-2-nitrobenzoic acid-coated silver nanoclusters (MNBA-AgNCs) to kill strains of Neisseria gonorrhoeae. Using an in vitro bactericidal assay, MNBA-AgNCs had been found to show significantly higher anti-gonococcal bioactivity than the antibiotics ceftriaxone and azithromycin and silver nitrate. These nanoclusters were effective against both planktonic bacteria and a gonococcal infection of human cell cultures in vitro. Treatment of human cells in vitro with MNBA-AgNCs did not induce significant release of lactate dehydrogenase, suggesting minimal cytotoxicity to eukaryotic cells. Our results suggest that MNBA-AgNCs hold great potential for topical treatment of localized gonorrhoeae.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanocluster Synthesis and Applications · COVID-19 Impact on Reproduction · Syphilis Diagnosis and Treatment
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Physics and Astronomy, Faculty of Physical Sciences and Engineering, University of Southampton, Southampton SO17 1BJ, U.K.
Neisseria Research Laboratory, Molecular Microbiology, School of Clinical and Experimental Sciences, Sir Henry Wellcome Laboratories, Faculty of Medicine, University of Southampton, Southampton SO16 6YD, U.K.
Department of Chemistry & Chemical Engineering, Syed Babar Ali School of Science and Engineering, Lahore University of Management Sciences (LUMS), DHA, Lahore Cantt 54792, Pakistan
Institute for Life Sciences, University of Southampton, Southampton SO17 1BJ, U.K.
Funding
Biotechnology and Biological Sciences Research Council
References (76)
Show source-provided references
1. 10.2471/BLT.18.22848610.2471/BLT.18.228486 ↗
2. 10.1146/annurev-micro-090816-09353010.1146/annurev-micro-090816-093530 ↗
3. Mayor M. T. · Am. Fam. Physician · 2012 · 86 · 931
4. 10.1016/j.idc.2013.08.00410.1016/j.idc.2013.08.004 ↗
5. 10.1128/CMR.00010-1410.1128/CMR.00010-14 ↗
6. 10.1016/S1473-3099(17)30753-310.1016/S1473-3099(17)30753-3 ↗
7. 10.2147/IJN.S12195610.2147/IJN.S121956 ↗
8. 10.1039/C7CS00748E10.1039/C7CS00748E ↗
9. 10.2147/IJN.S13452610.2147/IJN.S134526 ↗
10. 10.1016/j.apt.2010.11.01110.1016/j.apt.2010.11.011 ↗
11. 10.1021/acsnano.6b0420710.1021/acsnano.6b04207 ↗
12. 10.1016/j.addr.2013.07.01110.1016/j.addr.2013.07.011 ↗
13. 10.3389/fmicb.2018.0144110.3389/fmicb.2018.01441 ↗
14. 10.1016/j.biomaterials.2011.11.05710.1016/j.biomaterials.2011.11.057 ↗
15. 10.1021/nn504262510.1021/nn5042625 ↗
16. 10.1021/ja301167y10.1021/ja301167y ↗
17. Gupta A. · Nano Futures · 2017 · 1 · 01500410.1088/2399-1984/aa69fb ↗
18. 10.1038/cddis.2013.31410.1038/cddis.2013.314 ↗
19. 10.1088/0957-4484/25/13/13510110.1088/0957-4484/25/13/135101 ↗
20. 10.1002/anie.20120592310.1002/anie.201205923 ↗
21. 10.1089/sur.2008.994110.1089/sur.2008.9941 ↗
22. 10.1111/j.1365-2672.2012.05253.x10.1111/j.1365-2672.2012.05253.x ↗
23. 10.1007/s00253-009-2159-510.1007/s00253-009-2159-5 ↗
24. 10.1002/smll.20070095410.1002/smll.200700954 ↗
25. 10.3390/ma606229510.3390/ma6062295 ↗
26. 10.1016/j.nano.2015.11.01610.1016/j.nano.2015.11.016 ↗
27. 10.3390/nano1002029210.3390/nano10020292 ↗
28. 10.1166/jnn.2005.03410.1166/jnn.2005.034 ↗
29. 10.1021/acsnano.6b0386210.1021/acsnano.6b03862 ↗
30. 10.1038/nrmicro302810.1038/nrmicro3028 ↗
31. 10.1039/C3RA44507K10.1039/C3RA44507K ↗
32. 10.1039/C4NR04561K10.1039/C4NR04561K ↗
33. 10.1039/C4RA12054J10.1039/C4RA12054J ↗
34. 10.1039/C3CC47512C10.1039/C3CC47512C ↗
35. 10.1038/nature1252310.1038/nature12523 ↗
36. 10.1039/c3ta11785e10.1039/c3ta11785e ↗
37. 10.1007/s12274-013-0395-610.1007/s12274-013-0395-6 ↗
38. 10.1016/j.biomaterials.2014.05.00710.1016/j.biomaterials.2014.05.007 ↗
39. 10.1021/acsomega.8b0243810.1021/acsomega.8b02438 ↗
40. 10.1021/acsnano.6b0386210.1021/acsnano.6b03862 ↗
41. 10.1021/acsami.6b0067010.1021/acsami.6b00670 ↗
42. 10.1002/anie.20090029810.1002/anie.200900298 ↗
43. 10.1021/ja801173r10.1021/ja801173r ↗
44. 10.1039/C4NR03782K10.1039/C4NR03782K ↗
45. Crede C. · 1884
46. Ricord P. · 1938
47. Nishida H. · Pediatrics · 1975 · 56 · 36810.1542/peds.56.3.368 ↗
48. 10.12659/MSM.91070510.12659/MSM.910705 ↗
49. 10.1093/infdis/133.6.62110.1093/infdis/133.6.621 ↗
50. 10.1136/sti.73.5.37810.1136/sti.73.5.378 ↗
51. Unemo M. · 2019 · 3710.1007/978-1-4939-9496-0_3 ↗
52. Workowski K. A. · Morb. Mortal. Wkly. Rep. · 2015 · 64 · 1
53. 10.1056/NEJMc151275710.1056/NEJMc1512757 ↗
54. 10.2807/1560-7917.ES.2018.23.27.180032310.2807/1560-7917.ES.2018.23.27.1800323 ↗
55. Clinical and Laboratory Standards Institute (CLSI) · 2018
56. 10.1016/j.jpha.2015.11.00510.1016/j.jpha.2015.11.005 ↗
57. 10.1016/j.actbio.2007.11.00610.1016/j.actbio.2007.11.006 ↗
58. 10.1007/s13204-014-0381-710.1007/s13204-014-0381-7 ↗
59. 10.1016/j.ijbiomac.2014.05.02510.1016/j.ijbiomac.2014.05.025 ↗
60. Qian, A.Antimicrobial Gel of Nanosize Ag for Treating Female External Reproductive Tract Infection and Its Preparation Method. CN101305983, 2008.
61. 10.1080/10601325.2016.116600010.1080/10601325.2016.1166000 ↗
62. 10.1371/journal.pone.006479410.1371/journal.pone.0064794 ↗
63. 10.1016/0022-1759(88)90310-910.1016/0022-1759(88)90310-9 ↗
64. 10.1128/IAI.00644-0610.1128/IAI.00644-06 ↗
65. 10.1002/tox.2188010.1002/tox.21880 ↗
66. Zaidi, T. S.; Cywes-Bentley, C.; Roberts, C.; Vinacur, M.; Pier, G. B. Development of a Murine Model of Neisseria Gonorrhoeae Conjunctivitis and Evaluation of the Protective Efficacy of Antibody to the Surface Polysaccharide/Capsule, Poly-N-acetyl glucosamine (PNAG). American Society for Microbiology, February 12–14, Baltimore, Maryland, 2018.
67. 10.1128/IAI.67.11.5699-5708.199910.1128/IAI.67.11.5699-5708.1999 ↗
68. 10.1128/mBio.00041-1310.1128/mBio.00041-13 ↗
69. 10.1128/IAI.68.12.6526-6534.200010.1128/IAI.68.12.6526-6534.2000 ↗
70. 10.1002/j.1460-2075.1991.tb07649.x10.1002/j.1460-2075.1991.tb07649.x ↗
71. 10.1128/IAI.31.3.965-970.198110.1128/IAI.31.3.965-970.1981 ↗
72. 10.1046/j.1365-2958.2000.01694.x10.1046/j.1365-2958.2000.01694.x ↗
73. 10.1016/S0966-842X(00)01913-210.1016/S0966-842X(00)01913-2 ↗
74. 10.1038/227382a010.1038/227382a0 ↗
75. 10.1093/infdis/149.2.16610.1093/infdis/149.2.166 ↗
76. 10.1128/JB.85.6.1274-1279.196310.1128/JB.85.6.1274-1279.1963 ↗