DNA-Coated Gold Nanoparticles for the Detection of mRNA in Live Hydra Vulgaris Animals
Source abstract
Abstract
Advances in nanoparticle design have led to the development of nanoparticulate systems that can sense intracellular molecules, alter cellular processes, and release drugs to specific targets in vitro. In this work, we demonstrate that oligonucleotide-coated gold nanoparticles are suitable for the detection of mRNA in live Hydra vulgaris, a model organism, without affecting the animal's integrity. We specifically focus on the detection of Hymyc1 mRNA, which is responsible for the regulation of the balance between stem cell self-renewal and differentiation. Myc deregulation is found in more than half of human cancers, thus the ability to detect in vivo related mRNAs through innovative fluorescent systems is of outmost interest.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Marine Invertebrate Physiology and Ecology · Lipid Membrane Structure and Behavior · Erythrocyte Function and Pathophysiology
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Istituto di Scienze Applicate e Sistemi Intelligenti “E.Caianiello”, Consiglio Nazionale delle Ricerche, Pozzuoli 80078, Italy
Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Road, Oxford OX1 3TA, United Kingdom
Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez 43721, Egypt
Funding
Leverhulme Trust · H2020 Marie Sklodowska-Curie Actions · Biotechnology and Biological Sciences Research Council
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