journal article·2017-11-08

PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe

Xue Yang, Yanli Wen, Lele Wang, Chaoqun Zhou, Qian Li, Li Xu, Lanying Li, Jiye Shi, Ratnesh Lal, Shuzhen Ren, Jiang Li, Nengqin Jia, Gang Liu

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Abstract

A "sandwich-like" biosensor was developed on the basis of the magnetic bead platform for sensitive detection of breast cancer 1 (BRCA1) DNA. In the present study, a tetrahedron-structured reporter probe (TSRP) was designed, in which 3 vertices of the tetrahedron were labeled with digoxin (Dig), and the other one was labeled with a detection probe. TSRP here provided accurate enzyme loading and well-organized spatial arrangement for optimized signal amplification. The detection limit of this biosensor was as low as 10 fM, which is at least 4 orders of magnitude lower than that of the single DNA probe (100 pM), and the signal gain was 2 times higher than the analysis using three one-dimensional (1D) reporter probes. We could distinguish DNA sequences with only 1 base mismatch, and the performance of our TSRP biosensor was proven to be equally good in both PCR products and real fetal calf serum (FCS) sample as in buffer. We believe this work provided a novel avenue for the development of signal amplification strategies.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection · DNA and Nucleic Acid Chemistry

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Laboratory of Biometrology, Shanghai Institute of Measurement and Testing Technology, 1500 Zhangheng Road, Shanghai 201203, People’s Republic of China

Department of Chemistry, College of Life and Environmental Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, People’s Republic of China

Materials Science and Engineering Program, Department of Bioengineering, Department of Mechanical and Aerospace Engineering, Institute of Engineering in Medicine, University of California, San Diego, La Jolla, California 92093, United States

Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, People’s Republic of China

UCB Pharma, 208 Bath Road, Slough SL1 3WE, United Kingdom

Funding

Natural Science Foundation of Shanghai · Science and Technology Commission of Shanghai Municipality · National Natural Science Foundation of China

References (54)

Show source-provided references
1. 10.1039/C6AN01435F10.1039/C6AN01435F ↗
2. 10.1016/j.bios.2016.07.03010.1016/j.bios.2016.07.030 ↗
3. 10.1002/adma.20130133310.1002/adma.201301333 ↗
4. 10.1021/jacs.6b0567910.1021/jacs.6b05679 ↗
5. 10.1038/nnano.2011.14110.1038/nnano.2011.141 ↗
6. 10.1021/am200400910.1021/am2004009 ↗
7. 10.1002/adma.20140045110.1002/adma.201400451 ↗
8. 10.1038/nrclinonc.2015.7710.1038/nrclinonc.2015.77 ↗
9. 10.1021/am500883q10.1021/am500883q ↗
10. 10.1021/acsami.5b1211710.1021/acsami.5b12117 ↗
11. 10.1021/am200436610.1021/am2004366 ↗
12. 10.1021/acsami.7b0237710.1021/acsami.7b02377 ↗
13. 10.1126/science.108875510.1126/science.1088755 ↗
14. 10.1002/anie.20020160210.1002/anie.200201602 ↗
15. 10.1093/nar/gni08410.1093/nar/gni084 ↗
16. 10.1002/adma.20070191810.1002/adma.200701918 ↗
17. 10.1002/smll.20150235010.1002/smll.201502350 ↗
18. 10.1016/j.bios.2016.01.07310.1016/j.bios.2016.01.073 ↗
19. 10.1021/acssensors.7b0011910.1021/acssensors.7b00119 ↗
20. 10.1021/ac202335u10.1021/ac202335u ↗
21. 10.1016/j.bios.2016.05.02310.1016/j.bios.2016.05.023 ↗
22. 10.1021/am505913d10.1021/am505913d ↗
23. 10.1021/ac301924f10.1021/ac301924f ↗
24. 10.1039/C5AY02334C10.1039/C5AY02334C ↗
25. 10.1126/science.332.6034.114010.1126/science.332.6034.1140 ↗
26. 10.1038/nature0458610.1038/nature04586 ↗
27. 10.1002/adma.20100276710.1002/adma.201002767 ↗
28. 10.1039/c1cc11660f10.1039/c1cc11660f ↗
29. 10.1002/anie.20080426410.1002/anie.200804264 ↗
30. 10.1039/c3lc50629k10.1039/c3lc50629k ↗
31. 10.1038/am.2014.13110.1038/am.2014.131 ↗
32. 10.1021/acs.analchem.6b0447510.1021/acs.analchem.6b04475 ↗
33. 10.1002/anie.20161177710.1002/anie.201611777 ↗
34. 10.1021/jacs.7b0404010.1021/jacs.7b04040 ↗
35. 10.1021/ar400195t10.1021/ar400195t ↗
36. 10.1021/acsami.7b0364510.1021/acsami.7b03645 ↗
37. 10.1021/acsami.7b0319810.1021/acsami.7b03198 ↗
38. 10.1002/smll.20120277210.1002/smll.201202772 ↗
39. 10.1039/b402293a10.1039/b402293a ↗
40. 10.1126/science.112036710.1126/science.1120367 ↗
41. 10.1002/anie.20141072010.1002/anie.201410720 ↗
42. 10.1038/nprot.2016.07110.1038/nprot.2016.071 ↗
43. 10.1002/anie.20120235610.1002/anie.201202356 ↗
44. 10.1002/anie.20141089510.1002/anie.201410895 ↗
45. 10.1007/s11426-011-4327-610.1007/s11426-011-4327-6 ↗
46. 10.1007/s12033-007-0059-410.1007/s12033-007-0059-4 ↗
47. Ye D. K. · Prog. Chem. · 2017 · 29 · 36
48. 10.1021/am504773510.1021/am5047735 ↗
49. 10.1021/am501823q10.1021/am501823q ↗
50. Ford D. · Am. J. Hum. Genet. · 1995 · 57 · 1457
51. Easton D. F. · Am. J. Hum. Genet. · 1995 · 56 · 26510.1002/ajmg.1320560305 ↗
52. 10.1021/ac403726210.1021/ac4037262 ↗
53. 10.1073/pnas.051132510310.1073/pnas.0511325103 ↗
54. 10.1021/ja800554t10.1021/ja800554t ↗