Fast, Ultrasensitive Detection of Reactive Oxygen Species Using a Carbon Nanotube Based-Electrocatalytic Intracellular Sensor
Source abstract
Abstract
Herein, we report a highly sensitive electrocatalytic sensor-cell construct that can electrochemically communicate with the internal environment of immune cells (e.g., macrophages) via the selective monitoring of a particular reactive oxygen species (ROS), hydrogen peroxide. The sensor, which is based on vertically aligned single-walled carbon nanotubes functionalized with an osmium electrocatalyst, enabled the unprecedented detection of a local intracellular "pulse" of ROS on a short second time scale in response to bacterial endotoxin (lipopolysaccharide-LPS) stimulation. Our studies have shown that this initial pulse of ROS is dependent on NADPH oxidase (NOX) and toll like receptor 4 (TLR4). The results suggest that bacteria can induce a rapid intracellular pulse of ROS in macrophages that initiates the classical innate immune response of these cells to infection.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Electrochemical sensors and biosensors · Analytical Chemistry and Sensors · Advanced biosensing and bioanalysis techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom
Centre for Biomedical Research, School of Biomedical and Healthcare Science, Plymouth University, Drake Circus, Plymouth, Devon PL4 8AA, United Kingdom
School of Physics, The University of Melbourne, Victoria 3010, Australia
School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
Funding
Leverhulme Trust · European Research Council · Royal Society · Wellcome Trust · Biotechnology and Biological Sciences Research Council · Engineering and Physical Sciences Research Council
References (44)
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