journal article·2018-02-07

Engineered Phage Matrix Stiffness-Modulating Osteogenic Differentiation

Hee-Sook Lee, Jeong-In Kang, Woo-Jae Chung, Do Hoon Lee, Byung Yang Lee, Seung-Wuk Lee, So Young Yoo

Publisher page

Abstract

Herein, we demonstrate an engineered phage mediated matrix for osteogenic differentiation with controlled stiffness by cross-linking the engineered phage displaying Arg-Gly-Asp (RGD) and His-Pro-Gln (HPQ) with various concentrations of streptavidin or polymer, poly(diallyldimethylammonium)chloride (PDDA). Osteogenic gene expressions showed that they were specifically increased when MC3T3 cells were cultured on the stiffer phage matrix than the softer one. Our phage matrixes can be easily functionalized using chemical/genetic engineering and used as a stem cell tissue matrix stiffness platform for modulating differential cell expansion and differentiation.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Cellular Mechanics and Interactions · Monoclonal and Polyclonal Antibodies Research · Glycosylation and Glycoproteins Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Bioengineering, University of California, Berkeley, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States;

Ministry of Food and Drug Safety, Center for Test and Analysis, Busan 48562, Republic of Korea

BIO-IT Foundry Technology Institute, Pusan National University, Busan 46241, Republic of Korea

Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan 50612, Republic of Korea

Control and Instrumentation Engineering, Korea Maritime and Ocean University, Busan 49112, Republic of Korea

Genetic Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea

Mechanical Engineering, Korea University, Seoul 02841, Republic of Korea

Funding

Korea Health Industry Development Institute · National Research Foundation of Korea

References (47)

Show source-provided references
1. 10.1021/bm101347510.1021/bm1013475 ↗
2. 10.1021/la100226u10.1021/la100226u ↗
3. 10.1155/2014/19279010.1155/2014/192790 ↗
4. 10.1039/c0sm01220c10.1039/c0sm01220c ↗
5. 10.1021/nl803672810.1021/nl8036728 ↗
6. 10.1039/C7NR03332J10.1039/C7NR03332J ↗
7. 10.2174/1570193X120315042910541810.2174/1570193X1203150429105418 ↗
8. 10.1002/adhm.20150017910.1002/adhm.201500179 ↗
9. 10.2147/IJN.S7388310.2147/IJN.S73883 ↗
10. 10.1016/j.stem.2009.06.01610.1016/j.stem.2009.06.016 ↗
11. 10.1242/jcs.00606410.1242/jcs.006064 ↗
12. 10.1038/nmat405110.1038/nmat4051 ↗
13. 10.1111/joa.1224310.1111/joa.12243 ↗
14. 10.1016/J.ENG.2017.01.01410.1016/J.ENG.2017.01.014 ↗
15. 10.4252/wjsc.v7.i1.3710.4252/wjsc.v7.i1.37 ↗
16. 10.1007/s10529-005-1877-810.1007/s10529-005-1877-8 ↗
17. 10.1038/nature1051310.1038/nature10513 ↗
18. 10.1002/pros.106810.1002/pros.1068 ↗
19. 10.1016/S0022-2836(02)00844-610.1016/S0022-2836(02)00844-6 ↗
20. 10.1038/nrc274810.1038/nrc2748 ↗
21. 10.1385/1-59259-249-X:310.1385/1-59259-249-X:3 ↗
22. 10.1038/nbt0395-26510.1038/nbt0395-265 ↗
23. 10.1021/bc400323410.1021/bc4003234 ↗
24. 10.1039/C6NR09652B10.1039/C6NR09652B ↗
25. Koivunen E. · J. Biol. Chem. · 1993 · 268 · 2020510.1016/S0021-9258(20)80715-7 ↗
26. 10.1021/bi00154a00410.1021/bi00154a004 ↗
27. 10.1021/bi00047a00510.1021/bi00047a005 ↗
28. 10.1074/jbc.272.20.1322010.1074/jbc.272.20.13220 ↗
29. 10.1002/adma.20030481810.1002/adma.200304818 ↗
30. 10.1126/science.214303310.1126/science.2143033 ↗
31. 10.1021/bi00047a00610.1021/bi00047a006 ↗
32. 10.1002/adma.20030481810.1002/adma.200304818 ↗
33. 10.1016/0378-1119(93)90149-W10.1016/0378-1119(93)90149-W ↗
34. 10.1088/0031-9155/49/18/01410.1088/0031-9155/49/18/014 ↗
35. 10.1016/0021-9290(93)90042-D10.1016/0021-9290(93)90042-D ↗
36. 10.1002/jcp.2619310.1002/jcp.26193 ↗
37. 10.1371/journal.pone.017031210.1371/journal.pone.0170312 ↗
38. 10.1016/j.cell.2006.06.04410.1016/j.cell.2006.06.044 ↗
39. 10.1016/j.biomaterials.2016.05.00410.1016/j.biomaterials.2016.05.004 ↗
40. 10.1016/j.biomaterials.2012.12.00710.1016/j.biomaterials.2012.12.007 ↗
41. 10.1126/science.111699510.1126/science.1116995 ↗
42. 10.1371/journal.pone.013551910.1371/journal.pone.0135519 ↗
43. 10.1016/j.actbio.2016.06.03410.1016/j.actbio.2016.06.034 ↗
44. 10.1016/j.biomaterials.2012.10.05210.1016/j.biomaterials.2012.10.052 ↗
45. 10.1073/pnas.090326910710.1073/pnas.0903269107 ↗
46. 10.1016/j.biomaterials.2011.07.03510.1016/j.biomaterials.2011.07.035 ↗
47. 10.1016/j.actbio.2015.10.00410.1016/j.actbio.2015.10.004 ↗