journal article·2018-01-31Open access

High-Precision Photothermal Ablation Using Biocompatible Palladium Nanoparticles and Laser Scanning Microscopy

Belén Rubio-Ruiz, Ana M. Pérez-López, Thomas L. Bray, Martin Lee, Alan Serrels, Martín Prieto, Manuel Arruebo, Neil O. Carragher, Víctor Sebastián, Asier Unciti-Broceta

Abstract

Herein, we report a straightforward method for the scalable preparation of Pd nanoparticles (Pd-NPs) with reduced inherent cytotoxicity and high photothermal conversion capacity. These Pd-NPs are rapidly taken up by cells and able to kill labeled cancer cells upon short exposure to near-infrared (NIR) light. Following cell treatment with Pd-NPs, ablated areas were patterned with high precision by laser scanning microscopy, allowing one to perform cell migration assays with unprecedented accuracy. Using coherent Raman microscopy, cells containing Pd-NPs were simultaneously ablated and imaged. This novel methodology was combined with intravital imaging to mediate microablation of cancerous tissue in tumor xenografts in mice.
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Research context

OpenAlex topics: Nanoplatforms for cancer theranostics · Gold and Silver Nanoparticles Synthesis and Applications · Spectroscopy Techniques in Biomedical and Chemical Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Cancer Research UK Edinburgh Centre, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Crewe Road South, Edinburgh EH4 2XR, United Kingdom

MRC Centre for Inflammation Research, Queen’s Medical Research Institute, University of Edinburgh, Edinburgh EH16 4TJ, United Kingdom

Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Río Ebro-Edificio I+D, c/Poeta Mariano Esquillor s/n, 50018 Zaragoza, Spain

Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain

Funding

Royal Society · Universidad de Zaragoza · Engineering and Physical Sciences Research Council · College of Medicine and Veterinary Medicine, University of Edinburgh · Centro de Investigaci?n Biom?dica en Red en Bioingenier?a, Biomateriales y Nanomedicina · H2020 Marie Sklodowska-Curie Actions · Wellcome Trust · FP7 Ideas: European Research Council · Cancer Research UK

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