journal article·2013-06-12

Thiol-Reactive Molecule with Dual-Emission-Enhancement Property for Specific Prestaining of Cysteine Containing Proteins in SDS-PAGE

Yong Yu, Jie Li, Sijie Chen, Yuning Hong, Ka Ming Ng, Kathy Qian Luo, Ben Zhong Tang

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Abstract

1-[4-(Bromomethyl)phenyl]-1,2,2-triphenylethene (2) was synthesized and evaluated for specific fluorescent prestaining of proteins containing cysteine (Cys) in SDS-PAGE. The molecule showed classic aggregation-induced emission (AIE) property in protein labeling and its quantum efficiency was further enhanced upon reacting with Cys. The parameters of reaction such as labeling time and concentration of dye and reducing reagent-tris(2-carboxyethyl)phosphine (TCEP) were examined to obtain the optimal labeling condition. In addition to its specific labeling effect, molecule 2 also showed its advantage over traditional self-quenching dyes through labeling Cys containing BSA with different dye/Cys ratios.
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Research context

OpenAlex topics: Luminescence and Fluorescent Materials · Click Chemistry and Applications · Molecular Sensors and Ion Detection

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemical and Biomolecular Engineering, The Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China

Department of Chemistry, Institute for Advanced Study, State Key Laboratory of Molecular Neuroscience, Institute of Molecular Functional Materials and Division of Biomedical Engineering, Institute of Molecular Functional Materials, The Hong Kong University of Science and Technology (HKUST), Kowloon, Hong Kong, China

Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457

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