journal article·2021-09-08Open access

Wearable Collector for Noninvasive Sampling of SARS-CoV-2 from Exhaled Breath for Rapid Detection

Fernando Soto, Mehmet Ozgun Ozen, Carlos F. Guimarães, Jie Wang, Kallai Hokanson, Rajib Ahmed, Rui L. Reis, Ramasamy Paulmurugan, Utkan Demirci

ACS Applied Materials & Interfaces· Volume 13· Issue 35

Abstract

Airborne transmission of exhaled virus can rapidly spread, thereby increasing disease progression from local incidents to pandemics. Due to the COVID-19 pandemic, states and local governments have enforced the use of protective masks in public and work areas to minimize the disease spread. Here, we have leveraged the function of protective face coverings toward COVID-19 diagnosis. We developed a user-friendly, affordable, and wearable collector. This noninvasive platform is integrated into protective masks toward collecting airborne virus in the exhaled breath over the wearing period. A viral sample was sprayed into the collector to model airborne dispersion, and then the enriched pathogen was extracted from the collector for further analytical evaluation. To validate this design, qualitative colorimetric loop-mediated isothermal amplification, quantitative reverse transcription polymerase chain reaction, and antibody-based dot blot assays were performed to detect the presence of SARS-CoV-2. We envision that this platform will facilitate sampling of current SARS-CoV-2 and is potentially broadly applicable to other airborne diseases for future emerging pandemics.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: SARS-CoV-2 detection and testing · Biosensors and Analytical Detection · Infection Control and Ventilation

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Bio-Acoustic MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, School of Medicine Stanford University, Palo Alto, California 94304-5427, United States

Canary Center at Stanford for Cancer Early Detection, Department of Radiology, School of Medicine, Stanford University, Palo Alto, California 94304-5427, United States

3B’s Research Group—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães 4805-017, Portugal

ICVS/3B’s—Portuguese Government Associate Laboratory, University of Minho, Braga 4710-057, Portugal

Molecular Imaging Program at Stanford (MIPS), Department of Radiology, School of Medicine, Stanford University, Palo Alto, California 94304-5427, United States

Funding

Funda????o Luso-Americana para o Desenvolvimento · National Institutes of Health · Canary Foundation · Funda????o para a Ci??ncia e a Tecnologia · Precision Health and Integrated Diagnostics (PHIND) Center · International Alliance for Cancer Early Detection (ACED) · Rhodes Trust · Stanford RISE COVID-19 Crisis Response Trainee and Faculty Seed Grant Programs · Schmidt Science Fellows

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