journal article·2017-09-20

Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System

Xiangmeng Qu, Min Li, Hongbo Zhang, Chenglie Lin, Fei Wang, Mingshu Xiao, Yi Zhou, Jiye Shi, Ali Aldalbahi, Hao Pei, Hong Chen, Li Li

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Abstract

The development of a real-time continuous analytical platform for the pathogen detection is of great scientific importance for achieving better disease control and prevention. In this work, we report a rapid and recyclable microfluidic bioassay system constructed from oligonucleotide arrays for selective and sensitive continuous identification of DNA targets of fungal pathogens. We employ the thermal denaturation method to effectively regenerate the oligonucleotide arrays for multiple sample detection, which could considerably reduce the screening effort and costs. The combination of thermal denaturation and laser-induced fluorescence detection technique enables real-time continuous identification of multiple samples (<10 min per sample). As a proof of concept, we have demonstrated that two DNA targets of fungal pathogens (Botrytis cinerea and Didymella bryoniae) can be sequentially analyzed using our rapid microfluidic bioassay system, which provides a new paradigm in the design of microfluidic bioassay system and will be valuable for chemical and biomedical analysis.
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Research context

OpenAlex topics: Plant Pathogens and Fungal Diseases · Biosensors and Analytical Detection · Advanced Biosensing Techniques and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Pen-Tung Sah Institute of Micro-Nano Science and Technology of Xiamen University, Xiamen University, Xiamen 361005, P. R. China

School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, P. R. China

Department of Pharmaceutical Sciences Laboratory, Åbo Akademi University, Turku 20520, Finland

School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, P. R. China

Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China

UCB Pharma, 208 Bath Road, Slough SL1 3WE, U.K.

Chemistry Department, King Saud University, Riyadh 11451, Saudi Arabia

Funding

East China Normal University · China Postdoctoral Science Foundation · Natural Science Foundation of Fujian Province · Suomen Akatemia · Jane ja Aatos Erkon S??ti? · Science and Technology Commission of Shanghai Municipality · National Natural Science Foundation of China · Chinese Communist Party Central Committee · Fujian Province · Deanship of Scientific Research, College of Science Research Center, King Saud University

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