journal article·2017-05-17

Gelatin-Derived Graphene–Silicate Hybrid Materials Are Biocompatible and Synergistically Promote BMP9-Induced Osteogenic Differentiation of Mesenchymal Stem Cells

Yulong Zou, Nader Taheri Qazvini, Kylie Zane, Monirosadat Sadati, Qiang Wei, Junyi Liao, Jiaming Fan, Dongzhe Song, Jianxiang Liu, Chao Ma, Xiangyang Qu, Liqun Chen, Xinyi Yu, Zhicai Zhang, Chen Zhao, Zongyue Zeng, Ruyi Zhang, Shujuan Yan, Tingting Wu, Xingye Wu, Yi Shu, Yasha Li, Wenwen Zhang, Russell R. Reid, Michael J. Lee, Jennifer Moritis Wolf, Matthew Tirrell, Tong-Chuan He, Juan J. de Pablo, Zhong-Liang Deng

Publisher page

Abstract

Graphene-based materials are used in many fields but have found only limited applications in biomedicine, including bone tissue engineering. Here, we demonstrate that novel hybrid materials consisting of gelatin-derived graphene and silicate nanosheets of Laponite (GL) are biocompatible and promote osteogenic differentiation of mesenchymal stem cells (MSCs). Homogeneous cell attachment, long-term proliferation, and osteogenic differentiation of MSCs on a GL-scaffold were confirmed using optical microscopy and scanning electron microscopy. GL-powders made by pulverizing the GL-scaffold were shown to promote bone morphogenetic protein (BMP9)-induced osteogenic differentiation. GL-powders increased the alkaline phosphatase (ALP) activity in immortalized mouse embryonic fibroblasts but decreased the ALP activity in more-differentiated immortalized mouse adipose-derived cells. Note, however, that GL-powders promoted BMP9-induced calcium mineral deposits in both MSC lines, as assessed using qualitative and quantitative alizarin red assays. Furthermore, the expression of chondro-osteogenic regulator markers such as Runx2, Sox9, osteopontin, and osteocalcin was upregulated by the GL-powder, independent of BMP9 stimulation; although the powder synergistically upregulated the BMP9-induced Osterix expression, the adipogenic marker PPARγ was unaffected. Furthermore, in vivo stem cell implantation experiments demonstrated that GL-powder could significantly enhance the BMP9-induced ectopic bone formation from MSCs. Collectively, our results strongly suggest that the GL hybrid materials promote BMP9-induced osteogenic differentiation of MSCs and hold promise for the development of bone tissue engineering platforms.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Graphene and Nanomaterials Applications · Bone Tissue Engineering Materials · Mesenchymal stem cell research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Orthopaedic Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China

Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, Illinois 60637, United States

Institute for Molecular Engineering, The University of Chicago, Chicago, Illinois 60637, United States

Argonne National Laboratory, Argonne, Illinois 60439, United States

Ministry of Education Key Laboratory of Diagnostic Medicine, The Affiliated Hospitals of Chongqing Medical University, Chongqing 400016, China

Department of Conservative Dentistry and Endodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China

Department of Orthopaedic Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, China

Departments of Neurosurgery and Otolaryngology-Head & Neck Surgery, The Affiliated Zhongnan Hospital of Wuhan University, Wuhan 430071, China

Department of Laboratory Medicine and Clinical Diagnostics, the Affiliated Yantai Hospital, Binzhou Medical University, Yantai 264100, China

Department of Surgery, Section of Plastic Surgery, The University of Chicago Medical Center, Chicago, Illinois 60637, United States

Funding

Scoliosis Research Society · National Institute of Standards and Technology · National Institute of Dental and Craniofacial Research · National Center for Complementary and Integrative Health · U.S. Department of Defense · National Natural Science Foundation of China

References (54)

Show source-provided references
1. 10.1038/nm1295-132210.1038/nm1295-1322 ↗
2. 10.1089/ten.teb.2011.042710.1089/ten.teb.2011.0427 ↗
3. 10.1016/j.ijbiomac.2013.06.05410.1016/j.ijbiomac.2013.06.054 ↗
4. 10.1371/journal.pone.003242810.1371/journal.pone.0032428 ↗
5. 10.1371/journal.pone.009731610.1371/journal.pone.0097316 ↗
6. Lu S. · Am. J. Transl. Res. · 2016 · 8 · 3710
7. 10.1097/scs.000000000000171710.1097/scs.0000000000001717 ↗
8. 10.1073/pnas.95.5.250910.1073/pnas.95.5.2509 ↗
9. 10.1038/nprot.2007.13510.1038/nprot.2007.135 ↗
10. 10.2174/156652301334868910.2174/1566523013348689 ↗
11. 10.2106/00004623-200308000-0001710.2106/00004623-200308000-00017 ↗
12. 10.1038/gt.2014.4010.1038/gt.2014.40 ↗
13. 10.1016/j.biomaterials.2014.11.00710.1016/j.biomaterials.2014.11.007 ↗
14. 10.1111/j.1478-3231.2009.02111.x10.1111/j.1478-3231.2009.02111.x ↗
15. 10.1111/j.1582-4934.2008.00569.x10.1111/j.1582-4934.2008.00569.x ↗
16. 10.1111/jcmm.1209710.1111/jcmm.12097 ↗
17. 10.1002/jcb.2225410.1002/jcb.22254 ↗
18. 10.1371/journal.pone.009290810.1371/journal.pone.0092908 ↗
19. 10.1088/1748-6041/11/2/02502110.1088/1748-6041/11/2/025021 ↗
20. 10.1159/00044779710.1159/000447797 ↗
21. 10.1038/srep1752310.1038/srep17523 ↗
22. 10.1038/labinvest.2008.9810.1038/labinvest.2008.98 ↗
23. 10.7150/ijms.1333310.7150/ijms.13333 ↗
24. 10.1371/journal.pone.011306410.1371/journal.pone.0113064 ↗
25. 10.1002/jbmr.13310.1002/jbmr.133 ↗
26. 10.1074/jbc.m110.13051810.1074/jbc.m110.130518 ↗
27. 10.1093/nar/gks59610.1093/nar/gks596 ↗
28. 10.1371/journal.pone.013266610.1371/journal.pone.0132666 ↗
29. 10.3727/096368914x68105410.3727/096368914x681054 ↗
30. 10.1038/sj.gt.330229810.1038/sj.gt.3302298 ↗
31. 10.1159/00043859110.1159/000438591 ↗
32. 10.1038/onc.2011.9710.1038/onc.2011.97 ↗
33. 10.1159/00045688510.1159/000456885 ↗
34. 10.1089/scd.2013.058010.1089/scd.2013.0580 ↗
35. 10.1159/00043926810.1159/000439268 ↗
36. 10.1021/nn507488s10.1021/nn507488s ↗
37. Childres I. · New Dev. Photon Mater. Res. · 2013 · 1 · 1
38. Rey C. · 2014 · 22910.1007/978-3-642-53980-0_8 ↗
39. 10.18632/oncotarget.1467310.18632/oncotarget.14673 ↗
40. 10.1089/ten.tec.2015.024410.1089/ten.tec.2015.0244 ↗
41. 10.1002/jcb.1074410.1002/jcb.10744 ↗
42. 10.1074/jbc.m40334420010.1074/jbc.m403344200 ↗
43. 10.1074/jbc.m80638920010.1074/jbc.m806389200 ↗
44. 10.1002/adfm.20160310310.1002/adfm.201603103 ↗
45. 10.1039/c5nr07857a10.1039/c5nr07857a ↗
46. 10.1021/nl404640n10.1021/nl404640n ↗
47. 10.1039/c5ta01446h10.1039/c5ta01446h ↗
48. 10.1002/aenm.20140176110.1002/aenm.201401761 ↗
49. 10.1039/C3NR00803G10.1039/C3NR00803G ↗
50. 10.1073/pnas.081320010610.1073/pnas.0813200106 ↗
51. 10.1039/C2TB00274D10.1039/C2TB00274D ↗
52. 10.1016/j.carbon.2013.03.01010.1016/j.carbon.2013.03.010 ↗
53. 10.1021/nn202190c10.1021/nn202190c ↗
54. 10.1021/nn200500h10.1021/nn200500h ↗