Multifunctional Host Polymers Assist Au Nanoclusters Achieve High Quantum Yield and Mitochondrial Imaging

The high biocompatibility and excellent photostability of Au nanoclusters (AuNCs) make them stand out in the bioimaging of nanoparticles. However, the low quantum yield and inferior targeting ability of water-soluble AuNCs greatly limit their biological applications. In this study, we designed and synthesized multifunctional host polymers PolySC4AP and FGGC@AuNCs to fabricate PolySC4AP/FGGC@AuNC assemblies via a host–guest interaction based on SC4 (sulfonatocalix[4]arene) and positively charged FGGC ligands (phenylalanine-glycine-glycine-cysteine). Owing to the host–guest assembly strategy and rigid polymer matrix, the quantum yield of FGGC@AuNCs was significantly promoted from 7.0 to 35.3%, accompanied by considerable morphological changes of FGGC@AuNCs. Moreover, PolySC4AP/FGGC@AuNCs could monitor the location of mitochondria along with R (Pearson’s correlation coefficients) value for the co-localization as high as 0.9605, which provided a novel strategy for targeted bioimaging with luminophore.