Superwettable Microwell Arrays Constructed by Photocatalysis of Silver-Doped-ZnO Nanorods for Ultrasensitive and High-Throughput Electroanalysis of Glutathione in Hela Cells

Superwettable microwell arrays were constructed for the first time by the synergic photocatalysis of silver-doped-ZnO (Ag-ZnO) nanorods patterned on indium tin oxide (ITO) electrodes for electroanalysis of glutathione (GSH) in hela cells through the signal output of AgCl electrochemistry. The newly prepared Ag-ZnO nanorods with high photocatalysis were dispersed into an octadecyltrichlorosilane (OTS) matrix to be deposited onto ITO substrates, yielding superhydrophobic Ag-ZnO-OTS coatings. Superhydrophilic microwells were further created by the Ag-ZnO photocatalysis under UV irradiation to produce Ag-ZnO microwell arrays featuring the superwettability profile. The resulting Ag-ZnO microwell-modified ITO electrodes were employed further for electroanalysis of GSH through solid-state AgCl electrochemistry, in which the specific Ag-GSH interactions would trigger a rational decrease in the sharp AgCl peak currents at the potential approaching zero. Moreover, benefitting from the superwettability feature, the microwells on the ITO electrodes could facilitate the condensing enrichment of GSH analytes from the sample droplets, achieving improved analysis sensitivity. The as-developed electroanalysis strategy was subsequently demonstrated for the detection of GSH in hela cell supernatant with levels down to about 27.30 pM. Additionally, this synergic photocatalysis-based preparation route can be tailored for the large-scale fabrication of various array platforms with the superwettability feature for high-throughput and sensitive biological analysis.