DataVXACS Applied Materials & InterfacesPaper
journal article·2017-06-07

Preservation of DNA Nanostructure Carriers: Effects of Freeze–Thawing and Ionic Strength during Lyophilization and Storage

Bing Zhu, Yan Zhao, Jiangbing Dai, Jianbang Wang, Shu Xing, Linjie Guo, Nan Chen, Xiangmeng Qu, Li Li, Juwen Shen, Jiye Shi, Jiang Li, Lihua Wang

ACS Applied Materials & Interfaces· Volume 9· Issue 22
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Source abstract

Abstract

DNA nanostructures have attracted wide interest in biomedical applications, especially as nanocarriers for drug delivery. Therefore, it is important to ensure the structural integrity of DNA nanostructures under ambient temperature storage. In this study, we examined lyophilization-based preservation of DNA nanostructures by investigating the structural integrity of different DNA nanostructures reconstituted from lyophilization. We demonstrated that lyophilization under appropriate ionic strength is amenable to the storage of DNA nanostructures. Compared with that stored in liquid solution, DNA nanostructure carriers reconstituted from lyophilization showed significantly better structural integrity after an accelerated aging test equivalent to 100-day room-temperature storage.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: RNA Interference and Gene Delivery · Bacteriophages and microbial interactions · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility; CAS Key Laboratory of Interfacial Physics and Technology; Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

University of Chinese Academy of Sciences, Beijing 10049, China

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China

UCB Pharma, 208 Bath Road, Slough SL1 3WE, United Kingdom

Funding

National Natural Science Foundation of China

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