A Luminescent Cocaine Detection Platform Using a Split G-Quadruplex-Selective Iridium(III) Complex and a Three-Way DNA Junction Architecture
Source abstract
Abstract
In this study, a series of 10 in-house cyclometalated iridium(III) complexes bearing different auxiliary ligands were tested for their selectivity toward split G-quadruplex in order to construct a label-free switch-on cocaine detection platform employing a three-way junction architecture and a G-quadruplex motif as a signal output unit. Through two rounds of screening, we discovered that the iridium(III) complex 7 exhibited excellent selectivity toward the intermolecular G-quadruplex motif. A detection limit as low as 30 nM for cocaine can be achieved by this sensing approach with a linear relationship between luminescence intensity and cocaine concentration established from 30 to 300 nM. Furthermore, this sensing approach could detect cocaine in diluted oral fluid. We hope that our simple, signal-on, label-free oligonucleotide-based sensing method for cocaine using a three-way DNA junction architecture could act as a useful platform in bioanalytical research.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Criminal Justice and Penology · Social Issues and Policies in Latin America · Violence, Education, and Gender Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Chemistry, Hong Kong Baptist University, Hong Kong, China
Partner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Hong Kong, China
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
Funding
Health and Medical Research Fund · Hong Kong Baptist University · Fundo para o Desenvolvimento das Ciências e da Tecnologia · Research Grants Council, University Grants Committee, Hong Kong · Interdisciplinary Research Matching Scheme, Hong Kong Baptist University · Centre for Cancer and Inflammation Research, Hong Kong Baptist University · Agence Nationale de la Recherche · Universidade de Macau
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