Real-Time Imaging of Endocytosis and Intracellular Trafficking of Semiconducting Polymer Dots

Yuping Han; Xiaoming Li; Haobin Chen; Xingjie Hu; Yao Luo; Ting Wang; Zejun Wang; Qian Li; Chunhai Fan; Jiye Shi; Lihua Wang; Yun Zhao; Changfeng Wu; Nan Chen

doi:10.1021/acsami.7b05662

journal article·2017-06-28

Real-Time Imaging of Endocytosis and Intracellular Trafficking of Semiconducting Polymer Dots

Yuping Han, Xiaoming Li, Haobin Chen, Xingjie Hu, Yao Luo, Ting Wang, Zejun Wang, Qian Li, Chunhai Fan, Jiye Shi, Lihua Wang, Yun Zhao, Changfeng Wu, Nan Chen

ACS Applied Materials & Interfaces· Volume 9· Issue 25

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Source abstract

Abstract

Semiconducting polymer dots (Pdots) have shown great promise in biomedical applications, including biosensing, drug delivery, and live imaging of cells and biomolecules. Insight into the mechanism and regulation of cellular uptake and intracellular metabolism of Pdots is important for the development of superior Pdots-based theranostic nanoconjugates. Herein, we performed real-time imaging of endocytosis and intracellular trafficking of a type of fluorescent Pdots that showed excellent biocompatibility in various types of cells. The endocytic routes and kinetics of Pdots were differently regulated in distinct cell types. Following endocytosis, Pdots were transported in vesicles along microtubule and destined for lysosomes. Furthermore, our results revealed exosome-mediated extracellular release of Pdots and have tracked the dynamic process at the single particle level. These results provide new insight into the design of more effective and selective imaging probes as well as drug carriers.

Source: openalex_inverted_index · Quality: baseline

Research context

Polymers Nanomaterials Biomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Luminescence and Fluorescent Materials · Carbon and Quantum Dots Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

College of Life Sciences, Sichuan University, Chengdu 610064, China

School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China

Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China

Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

UCB Pharma, 208 Bath Road, Slough SL1 3WE, United Kingdom

Funding

Ministry of Science and Technology of the People's Republic of China · Youth Innovation Promotion Association of the Chinese Academy of Sciences · National Natural Science Foundation of China · Chinese Academy of Sciences · Central Committee of the Communist Party of China

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