DataVXACS Applied Materials & InterfacesPaper
journal article·2020-12-23Open access

Synthetic Extracellular Matrices as a Toolbox to Tune Stem Cell Secretome

Kaizheng Liu, Tomas Veenendaal, Maury Wiendels, Alejandra M. Ruiz-Zapata, Justin van Laar, Rafail Kyranas, Hilde Enting, Bram van Cranenbroek, Hans J. P. M. Koenen, Silvia M. Mihaila, Egbert Oosterwijk, Paul H. J. Kouwer

ACS Applied Materials & Interfaces· Volume 12· Issue 51
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Source abstract

Abstract

The application of stem cell-derived secretome in regenerative therapies offers the key advantage that instead of the stem cells, only their effective paracrine compounds are in vivo delivered. Ideally, the secretome can be steered by the culture conditions of the stem cells. So far, most studies use stem cells cultured on stiff plastic substrates, not representative of their native 3D environment. In this study, cells are cultured inside synthetic polyisocyanide (PIC)-based hydrogels, which are minimal, tailorable, and highly reproducible biomimetic matrices. Secretome analysis of human adipose-derived stem cells (multiplex, ELISA) displays that matrix manipulation is a powerful tool to direct the secretome composition. As an example, cells in nonadherent PIC gels secrete increased levels of IL-10 and the conditioned media from 3D culture accelerate wound closure. In all, our PIC-based approach opens the door to dedicated matrix design to engineer the secretome for custom applications.
Source: europepmc · Quality: candidate unverified · Source record ↗

Research context

Polymers

OpenAlex topics: 3D Printing in Biomedical Research · Graphene and Nanomaterials Applications · Electrospun Nanofibers in Biomedical Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands

Radboud Institute for Molecular Life Sciences, Department of Obstetrics and Gynecology, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands

Lab Medical Immunology, Laboratory Medicine, Radboud University Medical Centre, 6500 HB Nijmegen, The Netherlands

Utrecht Institute for Pharmaceutical Sciences, Division of Pharmacology, Utrecht University, 3854 CG Utrecht, The Netherlands

Radboud Institute for Molecular Life Sciences, Department of Urology, Radboud University Medical Centre, Geert Grooteplein 26-28, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands

Funding

Horizon 2020 Framework Programme

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