Self-Assembled Microgels for Sensitive and Low-Fouling Detection of Streptomycin in Complex Media

In terms of detection of antibiotics within complex media, the nonspecific adsorption is an enormous challenge and antifouling sensing interfaces capable of reducing the nonspecific adsorption from complex biological samples are highly desirable. In this work, a novel antifouling electrochemical immunosensor was explored based on the self-assembly of two kinds of poly(N-isopropylacrylamide) microgels on the surface of graphene oxide for sensitive detection of streptomycin (STR). The microgels modified with glycidyl methacrylate (GMA) and zwitterionic liquid 1-propyl-3-vinylimidazole sulfonate (PVIS) were prepared. The microgels with GMA were used by combining specific recognition of anti-STR. The rapid specific binding of antigen and anti-STR resulted in a decrease of current density to generate electrochemical responsive signals. Zwitterionic liquid-modified microgels were used for antifouling, which can form stronger hydration and show excellent antifouling ability. As a result, we achieved efficient and sensitive detection of STR in the complex sample with evidently resisted nonspecific adsorption effect, the wide linear range toward STR was from 0.05 to 100 ng mL–1, with a detection limit down to 1.7 pg mL–1. The immunosensor based on the surface functionalization of microgels showed promising applications for the detection of antibiotics in complex media.