DataVXACS Applied Materials & InterfacesPaper
journal article·2017-07-19

Combining Protein and miRNA Quantification for Bladder Cancer Analysis

Ruixue Duan, Zhenyu Zhang, Fuxin Zheng, Longwang Wang, Ju Guo, Tianchi Zhang, Xiaomeng Dai, Shengwei Zhang, Dong Yang, Renrui Kuang, Gongxian Wang, Chaohong He, Abdul Hakeem, Chang Shu, Ping Yin, Xiaoding Lou, Fuqing Zeng, Huageng Liang, Fan Xia

ACS Applied Materials & Interfaces· Volume 9· Issue 28
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Source abstract

Abstract

We combine the telomerase extension reaction and microRNA (miRNA)-induced rolling circle amplification, followed by graphene oxide (GO) and nicking enzyme-assisted signal amplification as a method to analyze telomerase and miRNA-21 in urine samples with the following merits. First, it is a binary assay and can simultaneously output double signals that correspond to the quantities of telomerase and miRNA, respectively. Second, telomerase activity is enhanced by using a DNA molecular beacon probe to inhibit the formation of G-quadruplex. Third, background noise is decreased significantly via introduction of GO. Fourth, performance tests on about 258 urine samples demonstrate that this binary assay can distinguish between urine from bladder cancer patients, those with cystitis, and normal individuals. Finally, this strategy also shows great potential in distinguishing between muscle-invasive bladder cancers and non-muscle-invasive bladder cancers. The proposed strategy will greatly contribute to clinical decision-making and individualized treatments.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · RNA Interference and Gene Delivery · Biosensors and Analytical Detection

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Urology, Union Hospital, Tongji Medical College, Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering and Department of Epidemiology and Biostatistics, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan 430074, China

Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China

Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China

Department of Urology, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China

Funding

Ministry of Science and Technology of the People's Republic of China · Huazhong University of Science and Technology · National Natural Science Foundation of China · Central People's Government of the People's Republic of China

References (32)

Show source-provided references
1. 10.1038/nrurol.2011.6910.1038/nrurol.2011.69 ↗
2. 10.1007/s00018-007-6481-810.1007/s00018-007-6481-8 ↗
3. 10.1038/nature0370210.1038/nature03702 ↗
4. 10.1038/nrc199710.1038/nrc1997 ↗
5. 10.1126/science.760542810.1126/science.7605428 ↗
6. 10.1021/ja106513f10.1021/ja106513f ↗
7. 10.1002/adma.20120041210.1002/adma.201200412 ↗
8. 10.1021/ac502436410.1021/ac5024364 ↗
9. 10.1038/ncomms209110.1038/ncomms2091 ↗
10. 10.1038/350718a010.1038/350718a0 ↗
11. 10.1073/pnas.05269809910.1073/pnas.052698099 ↗
12. 10.1093/nar/gni17810.1093/nar/gni178 ↗
13. 10.1261/rna.525060410.1261/rna.5250604 ↗
14. 10.1021/ja038822u10.1021/ja038822u ↗
15. 10.1021/ja311313b10.1021/ja311313b ↗
16. 10.1021/ja035848c10.1021/ja035848c ↗
17. 10.1002/anie.20090699210.1002/anie.200906992 ↗
18. 10.1093/nar/gkn102410.1093/nar/gkn1024 ↗
19. 10.1073/pnas.89.1.39210.1073/pnas.89.1.392 ↗
20. 10.1093/nar/gkq55610.1093/nar/gkq556 ↗
21. 10.1002/ange.20080566510.1002/ange.200805665 ↗
22. 10.1021/acsami.6b1221410.1021/acsami.6b12214 ↗
23. 10.1002/ange.20100282210.1002/ange.201002822 ↗
24. 10.1021/ar900245u10.1021/ar900245u ↗
25. 10.1002/anie.20090435910.1002/anie.200904359 ↗
26. 10.1039/b9nr00401g10.1039/b9nr00401g ↗
27. 10.1002/adfm.20090163910.1002/adfm.200901639 ↗
28. 10.1038/nchem.154810.1038/nchem.1548 ↗
29. 10.1016/j.eururo.2005.03.01410.1016/j.eururo.2005.03.014 ↗
30. 10.1038/nrurol.2013.11310.1038/nrurol.2013.113 ↗
31. 10.1038/nrc381710.1038/nrc3817 ↗
32. 10.1016/j.eururo.2005.09.00210.1016/j.eururo.2005.09.002 ↗