journal article·2017-11-01

Probing the Eumelanin–Silica Interface in Chemically Engineered Bulk Hybrid Nanoparticles for Targeted Subcellular Antioxidant Protection

Brigida Silvestri, Giuseppe Vitiello, Giuseppina Luciani, Vincenzo Calcagno, Aniello Costantini, Maria Gallo, Silvia Parisi, Simona Paladino, Mariagrazia Iacomino, Gerardino D’Errico, M. Federica Caso, Alessandro Pezzella, Marco d’Ischia

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Abstract

We disclose herein the first example of stable monodispersed hybrid nanoparticles (termed MelaSil-NPs) made up of eumelanin biopolymer intimately integrated into a silica nanoscaffold matrix and endowed with high antioxidant and cytoprotective effects associated with a specific subcellular localization. MelaSil-NPs have been fabricated by an optimized sol-gel methodology involving ammonia-induced oxidative polymerization of a covalent conjugate of the eumelanin building block 5,6-dihydroxyindole-2-carboxylic acid (DHICA) with 3-aminopropyltriethoxysilanes (APTS). They displayed a round-shaped (ca. 50-80 nm) morphology, exhibited the typical electron paramagnetic resonance signal of eumelanin biopolymers, and proved effective in promoting decomposition of hydrogen peroxide under physiologically relevant conditions. When administered to human ovarian cancer cells (A2780) or cervical cancer cells (HeLa), MelaSil-NPs were rapidly internalized and colocalized with lysosomes and exerted efficient protecting effects against hydrogen peroxide-induced oxidative stress and cytotoxicity.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: melanin and skin pigmentation · Phytochemicals and Antioxidant Activities · Synthesis of Organic Compounds

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemical, Materials and Production Engineering, University of Naples “Federico II”, p.le V. Tecchio 80, 80125 Naples, Italy

CSGI, Consorzio interuniversitario per lo sviluppo dei Sistemi a Grande Interfase, Sesto Fiorentino, via della Lastruccia 3, 50019 Firenze, Italy

Institute of Biophysics and Medical Physic, University of Leipzig, Härtelstraße 16-18, D−04107 Leipzig, Germany

Department of Molecular Medicine and Medical Biotechnology, University of Naples “‘Federico II”’, Naples, Via Pansini, 5, 80131 Naples, Italy

Ceinge Biotecnologie Avanzate, Via Gaetano Salvatore 486, 80145 Naples, Italy

Department of Chemical Sciences, University of Naples “Federico II”, Via Cintia 4, I-80126 Naples, Italy

Nanofaber Spin-off at Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Casaccia Research Centre, Via Anguillarese 301, 00123 Rome, Italy

National Interuniversity Consortium of Materials Science and Technology (INSTM), Florence 50121, Italy

Institute for Polymers, Composites and Biomaterials (IPCB), CNR, Via Campi Flegrei 34, I-80078 Pozzuoli (NA), Italy

Funding

Universit? degli Studi di Napoli Federico II

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