Sensitive and Specific Detection of l-Lactate Using an AIE-Active Fluorophore
Source abstract
Abstract
l-Lactate is a vital biomarker for many diseases and physiological fatigue. An AIE-active fluorophore (TPE-HPro) is combined with l-lactate oxidase (LOx) to determine l-lactate in aqueous fluid. The assay shows excellent sensitivity and anti-interference performance with a limit of detection (LOD) of 5.5 μM. In addition, sensitive detection of l-lactate is achieved even in a protein-rich environment. It is proposed that quantification of l-lactate be performed at 20 or 60 min in the current method. These characteristics endow the fluorometric assay with great potential for biomedical diagnostics.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Luminescence and Fluorescent Materials · Electrochemical sensors and biosensors · Molecular Sensors and Ion Detection
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
Funding
Innovation and Technology Commission
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