DataVXACS Applied Materials & InterfacesPaper
journal article·2017-02-08Open access

Virus-Inspired Self-Assembled Nanofibers with Aggregation-Induced Emission for Highly Efficient and Visible Gene Delivery

Chunqiu Zhang, Tingbin Zhang, Shubin Jin, Xiangdong Xue, Xiaolong Yang, Ningqiang Gong, Jinchao Zhang, Paul C. Wang, Jian-Hua Tian, Jinfeng Xing, Xing-Jie Liang

ACS Applied Materials & Interfaces· Volume 9· Issue 5
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Source abstract

Abstract

High-efficiency gene transfer and suitably low cytotoxicity are the main goals of gene transfection systems based on nonviral vectors. In addition, it is desirable to track the gene transfer process in order to observe and explain the mechanism. Herein, inspired by viral structures that are optimized for gene delivery, we designed a small-molecule gene vector (TR4) with aggregation-induced emission properties by capping a peptide containing four arginine residues with tetraphenylethene (TPE) and a lipophilic tail. This novel vector can self-assemble with plasmid DNA to form nanofibers in solution with low cytotoxicity, high stability, and high transfection efficiency. pDNA@TR4 complexes were able to transfect a variety of different cell lines, including stem cells. The self-assembly process induces bright fluorescence from TPE, which makes the nanofibers visible by confocal laser scanning microscopy (CLSM). This allows us for the tracking of the gene delivery process.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: RNA Interference and Gene Delivery · Advanced biosensing and bioanalysis techniques · Virus-based gene therapy research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology, No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China

School of Chemical Engineering and Technology, Tianjin University, No. 92, Weijin Road, Nankai District, Tianjin 300072, China

College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, 071002, China

Laboratory of Molecular Imaging, Department of Radiology, Howard University, Washington, DC 20060, United States

College of Science and Engineering, Fu Jen Catholic University, Taipei 24205, Taiwan

Funding

Ministry of Science and Technology of the People's Republic of China · Bureau of International Cooperation, Chinese Academy of Sciences · National Institute on Minority Health and Health Disparities · National Natural Science Foundation of China · Chinese Academy of Sciences

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