DataVXACS Applied Materials & InterfacesPaper
journal article·2015-08-26

Sensitive Detection of ssDNA Using an LRET-Based Upconverting Nanohybrid Material

Joe Gerald Jesu Raj, Marta Quintanilla, Khaled A. Mahmoud, Andy Ng, Fiorenzo Vetrone, Mohammed Zourob

ACS Applied Materials & Interfaces· Volume 7· Issue 33
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Source abstract

Abstract

Water-dispersible, optical hybrid nanoparticles are preferred materials for DNA biosensing due to their biocompatibility. Upconverting nanoparticles are highly desirable optical probes in sensors and bioimaging owing to their sharp emission intensity in the visible region. We herein report a highly sensitive ss-DNA detection based on an energy transfer system that uses a nanohybrid material synthesized by doping NaYF4:Tm(3+)/Yb(3+) upconverting nanoparticles (UCNPs) on silica coated polystyrene-co-acrylic acid (PSA) nanoparticles (PSA/SiO2) as the donor, and gold nanoparticles (AuNPs) decorated with Ir(III) complex as the acceptor. UCNPs tagged on PSA/SiO2 and the cyclometalated Ir(III)/AuNP conjugates were then linked through the ss-DNA sequence. Sequential addition of the target DNA to the probe molecular beacon complex resulted in the separation of the optical nanohybrid material and the quencher, leading to a measurable increase in the blue fluorescence emission intensity. Our results have shown a linear relationship between the fluorescence intensity and target DNA concentration down to the picomolar.
Source: openalex_inverted_index · Quality: baseline

Research context

NanomaterialsBiomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Quantum Dots Synthesis And Properties · Luminescence Properties of Advanced Materials

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Biosensors, Bio-MEMS, Bionanotechnology Laboratory (BBBL), Institute for Global Food Security, School of Biological Sciences, Queen’s University, Queen’s University Belfast, 18-30 Malone Road, Belfast, BT9 5BN, United Kingdom

Advanced Materials Laboratory, Institut National de la Recherche Scientifique - Énergie, Matériaux et Télécommunications, Université du Québec, Varennes, J3X 1S2 Québec, Canada

Qatar Environment and Energy Research Institute, Qatar Foundation, Doha, Qatar

Centre for Self-Assembled Chemical Structures, McGill University, 845 Sherbrooke Street West, Montreal H3A 0G4 Québec, Canada

Department of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Rd, Riyadh 11533, Saudi Arabia

Funding

Fonds de Recherche du Québec - Nature et Technologies · Natural Sciences and Engineering Research Council of Canada

References (27)

Show source-provided references
1. 10.1038/nbt76710.1038/nbt767 ↗
2. 10.1038/nmat96110.1038/nmat961 ↗
3. 10.1364/OE.18.02354410.1364/OE.18.023544 ↗
4. 10.1039/c0nr00116c10.1039/c0nr00116c ↗
5. 10.1002/ange.20100395910.1002/ange.201003959 ↗
6. 10.1039/b809132n10.1039/b809132n ↗
7. 10.1021/cm048016210.1021/cm0480162 ↗
8. 10.1002/anie.20050190710.1002/anie.200501907 ↗
9. 10.1021/ja064402410.1021/ja0644024 ↗
10. 10.1021/ja076151k10.1021/ja076151k ↗
11. 10.1016/j.bios.2011.07.05710.1016/j.bios.2011.07.057 ↗
12. 10.1021/ac201631b10.1021/ac201631b ↗
13. 10.1039/c2cc33289b10.1039/c2cc33289b ↗
14. 10.1021/ac901808q10.1021/ac901808q ↗
15. 10.1021/ac049787s10.1021/ac049787s ↗
16. 10.1021/nl035093810.1021/nl0350938 ↗
17. 10.1073/pnas.113202510010.1073/pnas.1132025100 ↗
18. 10.1038/8676210.1038/86762 ↗
19. 10.1021/ja025814p10.1021/ja025814p ↗
20. 10.1002/smll.20130376610.1002/smll.201303766 ↗
21. 10.1039/C0AN00444H10.1039/C0AN00444H ↗
22. 10.1039/c0cc04288a10.1039/c0cc04288a ↗
23. 10.1039/c1cc10544b10.1039/c1cc10544b ↗
24. 10.1021/ic901660m10.1021/ic901660m ↗
25. 10.1021/ja061848b10.1021/ja061848b ↗
26. 10.1364/OE.16.01378110.1364/OE.16.013781 ↗
27. 10.1016/j.colsurfa.2004.07.02310.1016/j.colsurfa.2004.07.023 ↗