DataVXACS Applied Materials & InterfacesPaper
journal article·2020-10-28

Label-Free Analysis of H5N1 Virus Based on Three-Segment Branched DNA-Templated Fluorescent Silver Nanoclusters

Ying Zhang, Fei Mu, Yefan Duan, Qi Li, Yating Pan, Hongfang Du, Panpan He, Xizhong Shen, Zhimin Luo, Changfeng Zhu, Lianhui Wang

ACS Applied Materials & Interfaces· Volume 12· Issue 43
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Source abstract

Abstract

Since H5N1 virus is a highly infectious pathogen that causes outbreaks of avian influenza, developing a sensitive and rapid diagnostic platform to sense it becomes significant. Here, a novel label-free fluorescence sensing platform based on DNA-templated silver nanoclusters (DNA-Ag NCs) is developed to detect the H5N1 gene sequence representing H5N1 virus. The three-segment-branched DNA structure with closed cytosine-rich loop is designed as an effective template to produce fluorescent Ag NCs, which is different with the previous design of cytosine-rich loop formed by hairpin-like single-stranded DNA or double-stranded DNA. The proposed fluorescence detection approach gives a wide linear range (500 pM-2 μM) and a low detection limit (500 pM) to sense H5N1 gene sequence. Furthermore, selective analysis of target DNA shows that our constructed analytical strategy has a high selectivity to H5N1 gene sequence. It is regarded as a promising method for highly sensitive and selective sensing of H5N1 virus.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Nanocluster Synthesis and Applications · Advanced biosensing and bioanalysis techniques · Advanced Nanomaterials in Catalysis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Key Laboratory for Organic Electronics and Information Displays (KLOEID) & Jiangsu Key Laboratory for Biosensor, Institute of Advanced Materials (IAM), Jiangsu National Synergistic Innovation Center for Advanced Materials (SICAM), College of Electronic and optical Engineering & College of Microelectronic, Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China

Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai 200032, China

Funding

Ministry of Education of the People's Republic of China · Ministry of Science and Technology of the People's Republic of China · Government of Jiangsu Province · China Scholarship Council · Ministry of Human Resources and Social Security · National Natural Science Foundation of China · Key Grant for Special Professors in Jiangsu Province · Key Research and Development Program of Jiangsu · Natural Science Key Fund for Colleges and Universities in Jiangsu Province

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