DataVXACS Applied Materials & InterfacesPaper
journal article·2016-11-02Open access

Ordered Silicon Pillar Arrays Prepared by Electrochemical Micromachining: Substrates for High-Efficiency Cell Transfection

Frances J. Harding, Salvatore Surdo, Bahman Delalat, Chiara Cozzi, Roey Elnathan, Stan Gronthos, Nicolas H. Voelcker, Giuseppe Barillaro

ACS Applied Materials & Interfaces· Volume 8· Issue 43
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Source abstract

Abstract

Ordered arrays of silicon nano- to microscale pillars are used to enable biomolecular trafficking into primary human cells, consistently demonstrating high transfection efficiency can be achieved with broader and taller pillars than reported to date. Cell morphology on the pillar arrays is often strikingly elongated. Investigation of the cellular interaction with the pillar reveals that cells are suspended on pillar tips and do not interact with the substrate between the pillars. Although cells remain suspended on pillar tips, acute local deformation of the cell membrane was noted, allowing pillar tips to penetrate the cell interior, while retaining cell viability.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Microfluidic and Bio-sensing Technologies · Nanowire Synthesis and Applications · Neuroscience and Neural Engineering

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, Mawson Lakes, University of South Australia, Adelaid, South Australia 5095, Australia

Dipartimento di Ingegneria dell’Informazione, Università di Pisa, via G. Caruso 16, 56122 Pisa, Italy

South Australian Health and Medical Research Institute, Adelaide 5005, South Australia, Australia

Mesenchymal Stem Cell Group Laboratory, School of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, South Australia, Australia

Funding

Ministero dell’Istruzione, dell’Università e della Ricerca

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