Thiol-Epoxy “Click” Chemistry to Engineer Cytocompatible PEG-Based Hydrogel for siRNA-Mediated Osteogenesis of hMSCs

Cong Truc Huynh; Fangze Liu; Yuxuan Cheng; Katherine A. Coughlin; Eben Alsberg

doi:10.1021/acsami.8b07167

journal article·2018-08-08

Thiol-Epoxy “Click” Chemistry to Engineer Cytocompatible PEG-Based Hydrogel for siRNA-Mediated Osteogenesis of hMSCs

Cong Truc Huynh, Fangze Liu, Yuxuan Cheng, Katherine A. Coughlin, Eben Alsberg

ACS Applied Materials & Interfaces· Volume 10· Issue 31

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Source abstract

Abstract

Thiol-epoxy "click" chemistry is employed for the first time to engineer a new cytocompatible PEG-based hydrogel system in aqueous media with the ability to encapsulate human mesenchymal stem cells (hMSCs) and control their fate for tissue regeneration. Cells were easily encapsulated into the hydrogels and exhibited high cell viability over 4 weeks of culture regardless of the presence of siRNA, complexed with polyethylenimine (PEI) in the form of siRNA/PEI nanocomplexes, indicating the biocompatibility of the developed hydrogel. Loading pro-osteogenic siNoggin in the hydrogel significantly enhanced the osteogenesis of encapsulated hMSCs, demonstrating the potential application of this system in tissue engineering.

Source: openalex_inverted_index · Quality: baseline

Research context

Polymers Nanomaterials Biomaterials

OpenAlex topics: RNA Interference and Gene Delivery · Hydrogels: synthesis, properties, applications · Supramolecular Self-Assembly in Materials

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, United States

Department of Biomedical Engineering, Northwestern University, Evanston, Illinois 60208, United States

Department of Orthopaedic Surgery, Case Western Reserve University, Cleveland, Ohio 44106, United States

Funding

National Institute of Arthritis and Musculoskeletal and Skin Diseases

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