DataVXACS Applied Materials & InterfacesPaper
journal article·2016-04-20

Self-Assembled Fluorescent Bovine Serum Albumin Nanoprobes for Ratiometric pH Measurement inside Living Cells

Qiaoyu Yang, Zhongju Ye, Meile Zhong, Bo Chen, Jian Chen, Rongjin Zeng, Lin Wei, Hung-wing Li, Lehui Xiao

ACS Applied Materials & Interfaces· Volume 8· Issue 15
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Source abstract

Abstract

In this work, we demonstrated a new ratiometric method for the quantitative analysis of pH inside living cells. The structure of the nanosensor comprises a biofriendly fluorescent bovine serum albumin (BSA) matrix, acting as a pH probe, and pH-insensitive reference dye Alexa 594 enabling ratiometric quantitative pH measurement. The fluorescent BSA matrix was synthesized by cross-linking of the denatured BSA proteins in ethanol with glutaraldehyde. The size of the as-synthesized BSA nanoparticles can be readily manipulated from 30 to 90 nm, which exhibit decent fluorescence at the peak wavelength of 535 nm with a pH response range of 6-8. The potential of this pH sensor for intracellular pH monitoring was demonstrated inside living HeLa cells, whereby a significant change in fluorescence ratio was observed when the pH of the cell was switched from normal to acidic with anticancer drug treatment. The fast response of the nanosensor makes it a very powerful tool in monitoring the processes occurring within the cytosol.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Molecular Sensors and Ion Detection · Carbon and Quantum Dots Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Dynamic Optical Microscopic Imaging Laboratory, Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Key Laboratory of Phytochemical R&D of Hunan Province, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, Hunan 410081, People’s Republic of China

Key Laboratory of Theoretical Organic Chemistry and Function Molecule of Ministry of Education, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, Hunan 411201, People’s Republic of China

Department of Chemistry, Hongkong Baptist University, Kowloon Tong, Hong Kong, People’s Republic of China

Funding

Program for New Century Excellent Talents in University · Natural Science Foundation of Hunan Province · China Postdoctoral Science Foundation · National Natural Science Foundation of China

References (22)

Show source-provided references
1. 10.1038/nrc311010.1038/nrc3110 ↗
2. 10.1038/nrm282010.1038/nrm2820 ↗
3. 10.3389/fphys.2013.0037010.3389/fphys.2013.00370 ↗
4. 10.1002/anie.20140574210.1002/anie.201405742 ↗
5. 10.1038/sj.cdd.440146610.1038/sj.cdd.4401466 ↗
6. 10.1021/ja809149g10.1021/ja809149g ↗
7. 10.1088/2050-6120/2/4/04200110.1088/2050-6120/2/4/042001 ↗
8. 10.1021/cr900249z10.1021/cr900249z ↗
9. 10.1021/ac103140x10.1021/ac103140x ↗
10. 10.1021/ja909570v10.1021/ja909570v ↗
11. 10.1021/nn402581q10.1021/nn402581q ↗
12. 10.1021/cm052750j10.1021/cm052750j ↗
13. 10.1016/B978-0-12-420138-5.00023-910.1016/B978-0-12-420138-5.00023-9 ↗
14. 10.1039/C4CS00280F10.1039/C4CS00280F ↗
15. 10.1021/ja404900k10.1021/ja404900k ↗
16. 10.1021/cm500366910.1021/cm5003669 ↗
17. 10.1146/annurev.biochem.67.1.50910.1146/annurev.biochem.67.1.509 ↗
18. 10.3390/s13121673610.3390/s131216736 ↗
19. 10.1021/bi026609p10.1021/bi026609p ↗
20. 10.1021/nl034733410.1021/nl0347334 ↗
21. 10.1039/b901966a10.1039/b901966a ↗
22. 10.1529/biophysj.106.08896310.1529/biophysj.106.088963 ↗