journal article·2014-08-13

Stretchable and Micropatterned Membrane for Osteogenic Differentation of Stem Cells

Xuetao Shi, Lei Li, Serge Ostrovidov, Yiwei Shu, Ali Khademhosseini, Hongkai Wu

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Abstract

Stem cells have emerged as potentially useful cells for regenerative medicine applications. To fully harness this potential, it is important to develop in vitro cell culture platforms with spatially regulated mechanical, chemical, and biological cues to induce the differentiation of stem cells. In this study, a cell culture platform was constructed that used polydopamine (PDA)-coated parafilm. The modified parafilm supports cell attachment and proliferation. In addition, because of the superb plasticity and ductility of the parafilm, it can be easily micropatterned to regulate the spatial arrangements of cells, and can exert different mechanical tensions. Specifically, we constructed a PDA-coated parafilm with grooved micropatterns to induce the osteogenic differentiation of stem cells. Adipose-derived mesenchymal stem cells that were cultured on the PDA-coated parafilm exhibited significantly higher osteogenic commitment in response to mechanical and spatial cues compared to the ones without stretch. Our findings may open new opportunities for inducing osteogenesis of stem cells in vitro using the platform that combines mechanical and spatial cues.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Polymer Surface Interaction Studies · 3D Printing in Biomedical Research · Bone Tissue Engineering Materials

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8578, Japan

Beijing Key Laboratory of Cryo-Biomedical Engineering and Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China

Department of Chemistry, Hong Kong University of Science & Technology, Hong Kong, China

Center for Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, United States

Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea

Department of Physics, King Abdulaziz University, Jeddah 22254, Saudi Arabia

Funding

Research Grants Council, University Grants Committee, Hong Kong · Ministry of Education, Culture, Sports, Science, and Technology · Japan Society for the Promotion of Science

References (62)

Show source-provided references
1. Passier R. · Nature · 2008 · 453 · 32210.1038/nature07040 ↗
2. Bianco P. · Nature · 2001 · 414 · 11810.1038/35102181 ↗
3. Rosenzweig A. · N. Engl. J. Med. · 2006 · 355 · 127410.1056/NEJMe068172 ↗
4. Brinster R. L. · Science · 2002 · 296 · 217410.1126/science.1071607 ↗
5. Brunet J. L. · Science · 1998 · 280 · 145510.1126/science.280.5368.1455 ↗
6. Kirouac D. C. · Cell Stem Cell · 2008 · 3 · 36910.1016/j.stem.2008.09.001 ↗
7. Boheler K. R. · Circ. Res. · 2002 · 91 · 18910.1161/01.RES.0000027865.61704.32 ↗
8. Weiner S. · Annu. Rev. Mater. Sci. · 1998 · 28 · 27110.1146/annurev.matsci.28.1.271 ↗
9. Ochareon P. · Anat. Rec. · 2007 · 290 · 136610.1002/ar.20588 ↗
10. Aaron J. E. · Bone Miner. · 1994 · 25 · 21110.1016/S0169-6009(08)80240-1 ↗
11. Parfitt A. M. · J. Bone Miner. Res. · 2002 · 17 · 174110.1359/jbmr.2002.17.10.1741 ↗
12. Turner R. T. · Endocr. Rev. · 1994 · 15 · 275
13. Ushiku C. · J. Orthop. Res. · 2010 · 28 · 133810.1002/jor.21105 ↗
14. Einhorn T. A. · Clin. Orthop. Relat. Res. · 1998 · 355 · S710.1097/00003086-199810001-00003 ↗
15. Shi X. · Adv. Healthcare Mater. · 2013 · 2 · 122910.1002/adhm.201300012 ↗
16. Shi X. · Adv. Healthcare Mater. · 2013 · 2 · 84610.1002/adhm.201200333 ↗
17. Guvendiren M. · Adv. Healthcare Mater. · 2013 · 2 · 15510.1002/adhm.201200105 ↗
18. Discher D. E. · Science · 2005 · 310 · 113910.1126/science.1116995 ↗
19. Discher D. E. · Science · 2009 · 324 · 167310.1126/science.1171643 ↗
20. Engler A. J. · Cell · 2006 · 126 · 67710.1016/j.cell.2006.06.044 ↗
21. Eyckmas J. · Nat. Mater. · 2014 · 13 · 54210.1038/nmat3989 ↗
22. Chen C. S. · J. Cell. Sci. · 2008 · 121 · 328510.1242/jcs.023507 ↗
23. Augustin G. · Injury · 2007 · 38 · 111510.1016/j.injury.2007.05.017 ↗
24. Zamanian B. · Small · 2010 · 6 · 93710.1002/smll.200902326 ↗
25. Proksch S. · Cell Tissue Res. · 2014 · 356 · 15910.1007/s00441-013-1777-5 ↗
26. Zhang J.-T. · Adv. Funct. Mater. · 2011 · 21 · 407910.1002/adfm.201100739 ↗
27. Tang L. · Biochem. Biophys. Res. Commun. · 2006 · 344 · 12210.1016/j.bbrc.2006.03.123 ↗
28. Foolen J. · J. Orthop. Res. · 2008 · 26 · 126310.1002/jor.20586 ↗
29. Glucksmann A. · J. Anat. · 1942 · 76 · 231
30. Shi X. · ACS Appl. Mater. Interfaces · 2013 · 5 · 1058310.1021/am402388k ↗
31. Paz A. C. · Lab Chip · 2011 · 11 · 344010.1039/c1lc20506d ↗
32. Ren K. · Proc. Natl. Acad. Sci. U.S.A. · 2011 · 108 · 816210.1073/pnas.1100356108 ↗
33. Dai W. · Biomicrofluidics · 2010 · 4 · 02410110.1063/1.3398319 ↗
34. Lee H. · Science · 2007 · 318 · 42610.1126/science.1147241 ↗
35. Ryu J. · Adv. Funct. Mater. · 2010 · 20 · 213210.1002/adfm.200902347 ↗
36. Lee H. · Nature · 2007 · 448 · 33810.1038/nature05968 ↗
37. Yang Z. · Adv. Healthcare Mater. · 2012 · 1 · 54810.1002/adhm.201200073 ↗
38. Yan J. · Small · 2013 · 9 · 59610.1002/smll.201201064 ↗
39. Zheng W. · Adv. Healthcare Mater. · 2013 · 2 · 9510.1002/adhm.201200104 ↗
40. Kim S. · Adv. Funct. Mater. · 2013 · 23 · 1010.1002/adfm.201201994 ↗
41. Lee H. · Adv. Mater. · 2009 · 21 · 43110.1002/adma.200801222 ↗
42. Lynge M. E. · Nanoscale · 2011 · 3 · 491610.1039/c1nr10969c ↗
43. Kurpinski K. · Proc. Natl. Acad. Sci. U.S.A. · 2006 · 103 · 1609510.1073/pnas.0604182103 ↗
44. Kolind K. · Biomaterials · 2012 · 33 · 662610.1016/j.biomaterials.2012.05.070 ↗
45. Wang C. · Biotechnol. Prog. · 2008 · 24 · 114210.1002/btpr.52 ↗
46. Sengupta D. · Adv. Healthcare Mater. · 2012 · 1 · 78510.1002/adhm.201200195 ↗
47. Kanda K. · Cell Transplant. · 1993 · 2 · 47510.1177/096368979300200606 ↗
48. Aubin H. · Biomaterials · 2010 · 31 · 694110.1016/j.biomaterials.2010.05.056 ↗
49. Shi X. T. · Pharm. Res. · 2009 · 26 · 42210.1007/s11095-008-9759-0 ↗
50. Wang Y. · J. Biomater. Sci. Polym. Ed. · 2010 · 21 · 122710.1163/092050609X12481751806259 ↗
51. Shi X. T. · Langmuir · 2014 · 30 · 83210.1021/la4041216 ↗
52. Chien C.-Y. · ACS Appl. Mater. Interfaces · 2013 · 5 · 697510.1021/am401071f ↗
53. Rivera J. G. · J. Sep. Sci. · 2012 · 35 · 151410.1002/jssc.201200073 ↗
54. Fletcher D. A. · Nature · 2010 · 463 · 48510.1038/nature08908 ↗
55. Wang N. · Science · 2003 · 260 · 112410.1126/science.7684161 ↗
56. Chen N. · J. Mater. Chem. B · 2013 · 1 · 256810.1039/c3tb20153h ↗
57. Shi X. · Adv. Funct. Mater. · 2012 · 22 · 379910.1002/adfm.201200914 ↗
58. Namgung S. · ACS Nano · 2011 · 5 · 738310.1021/nn2023057 ↗
59. Higuchi A. · Chem. Rev. · 2013 · 113 · 329710.1021/cr300426x ↗
60. Klebe R. J. · Matrix · 1989 · 9 · 45110.1016/S0934-8832(11)80014-4 ↗
61. Matsugaki A. · Acta Biomater. · 2013 · 9 · 722710.1016/j.actbio.2013.03.015 ↗
62. Shi X. · Adv. Mater. · 2014 · 26 · 329010.1002/adma.201305804 ↗