Antibacterial Coatings on Titanium Surfaces: A Comparison Study Between in Vitro Single-Species and Multispecies Biofilm
Source abstract
Abstract
Dental plaque is a biofilm that causes dental caries, gingivitis, and periodontitis. Most of the studies in antibacterial coatings have been conducted by in vitro single-species biofilm formation, but oral biofilm involves more than 700 different bacterial species that are able to interact. Therefore, new studies are focused on in vitro multispecies biofilm models that mimic in vivo biofilms. The aim of the present work was to study different antibacterial coatings onto titanium surfaces and evaluate the in vitro antimicrobial properties of the surfaces on two different bacterial species and an oral biofilm. The lactate dehydrogenase assay determined that treated samples did not affect fibroblast viability. In addition, the viability of microorganisms on modified samples was evaluated by a LIVE/DEAD BacLight bacterial viability kit. Although a decrease in viable bacteria onto treated samples was obtained, the results showed differences in effectiveness when single-biofilm and oral plaque were tested. It confirms, as we expected, the distinct sensitivities that bacterial strains have. Thus, this multispecies biofilms model holds a great potential to assess antibacterial properties onto samples for dental purposes.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Oral microbiology and periodontitis research · Dental materials and restorations · Bacterial biofilms and quorum sensing
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), ETSEIB, Av. Diagonal 647, 08028, Barcelona, Spain
Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3-5, Pabellón 11, 28029 Madrid, Spain
Centre for Research in NanoEngineering (CRNE)-UPC, C/Pascual i Vila 15, 08028, Barcelona, Spain
Division of Endodontics, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, Hubei 430079, PR China
Funding
Fundación Ramón Areces · Canada Foundation for Innovation · Ministerio de Economía y Competitividad · National Natural Science Foundation of China · European Regional Development Fund
References (67)
Show source-provided references
1. Algraffee H. · Br. J. Oral Maxillofac. Surg. · 2012 · 50 · 68910.1016/j.bjoms.2011.11.020 ↗
2. Klinge B. · Dent. Clin. North Am. · 2005 · 49 · 66110.1016/j.cden.2005.03.007 ↗
3. Foster J. S. · Appl. Environ. Microbiol. · 2004 · 70 · 434010.1128/AEM.70.7.4340-4348.2004 ↗
4. Kolenbrander P. E. · Microbiol. Mol. Biol. Rev. · 2002 · 66 · 48610.1128/MMBR.66.3.486-505.2002 ↗
5. Sánchez M. C. · Dent. Mater. · 2014 · 30 · 116110.1016/j.dental.2014.07.008 ↗
6. Benítez-Páez A. · BMC Genomics · 2014 · 15 · 31110.1186/1471-2164-15-311 ↗
7. Bjarnsholt T. · APMIS, Suppl. · 2013 · 110.1111/apm.12099 ↗
8. Blanc V. · Periodontal Res. · 2014 · 49 · 32310.1111/jre.12110 ↗
9. Takahashi N. · Int. Congr. Ser. · 2005 · 1284 · 10310.1016/j.ics.2005.06.071 ↗
10. Grössner-Schreiber B. · Clin. Oral Implants Res. · 2009 · 20 · 81710.1111/j.1600-0501.2009.01729.x ↗
11. Pham L. C. · Arch. Oral Biol. · 2009 · 54 · 13210.1016/j.archoralbio.2008.09.007 ↗
12. Shen Y. · J. Endod. · 2011 · 37 · 65710.1016/j.joen.2011.02.007 ↗
13. Shen Y. · J. Endod. · 2010 · 36 · 182010.1016/j.joen.2010.08.029 ↗
14. Shen Y. · J. Endod. · 2009 · 35 · 98110.1016/j.joen.2009.04.030 ↗
15. Atiyeh B. S. · Burns J. Int. Soc. Burn Inj. · 2007 · 33 · 13910.1016/j.burns.2006.06.010 ↗
16. DeVasConCellos P. · Mater. Sci. Eng., C · 2012 · 32 · 111210.1016/j.msec.2012.02.020 ↗
17. 10.1111/clr.1242210.1111/clr.12422 ↗
18. Brook L. A. · J. Photochem. Photobiol. Chem. · 2007 · 187 · 5310.1016/j.jphotochem.2006.09.014 ↗
19. Chen W. · Biomaterials · 2006 · 27 · 551210.1016/j.biomaterials.2006.07.003 ↗
20. Godoy-Gallardo M. · Acta Biomater. · 2014 · 10 · 352210.1016/j.actbio.2014.03.026 ↗
21. Chen X. · Colloids Surf., B: Biointerfaces · 2013 · 107 · 18910.1016/j.colsurfb.2013.02.005 ↗
22. Li X. · Acta Biomater. · 2014 · 10 · 25810.1016/j.actbio.2013.09.009 ↗
23. Xie Y. · Composites, Part A: Appl. Sci. Manuf. · 2010 · 41 · 80610.1016/j.compositesa.2010.03.005 ↗
24. Bauer S. · Prog. Mater. Sci. · 2013 · 58 · 26110.1016/j.pmatsci.2012.09.001 ↗
25. Ahmed W. · 2012
26. Costa F. · Acta Biomater. · 2011 · 7 · 143110.1016/j.actbio.2010.11.005 ↗
27. Onaizi S. A. · Biotechnol. Adv. · 2011 · 29 · 6710.1016/j.biotechadv.2010.08.012 ↗
28. Holmberg K. V. · Acta Biomater. · 2013 · 9 · 822410.1016/j.actbio.2013.06.017 ↗
29. Chen R. · Acta Biomater. · 2012 · 8 · 437110.1016/j.actbio.2012.07.029 ↗
30. Pasupuleti M. · Crit. Rev. Biotechnol. · 2012 · 32 · 14310.3109/07388551.2011.594423 ↗
31. Reddy K. V. R. · Int. J. Antimicrob. Agents · 2004 · 24 · 53610.1016/j.ijantimicag.2004.09.005 ↗
32. Bouchet A. M. · Colloids Surf., B: Biointerfaces · 2014 · 114 · 36310.1016/j.colsurfb.2013.10.025 ↗
33. Jenssen H. · Biochimie · 2009 · 91 · 1910.1016/j.biochi.2008.05.015 ↗
34. Kirkpatrick C. H. · J. Infect. Dis. · 1971 · 124 · 53910.1093/infdis/124.6.539 ↗
35. Kim H. M. · J. Mater. Sci. Mater. Med. · 1997 · 8 · 34110.1023/A:1018524731409 ↗
36. Xiao S. J. · J. Mater. Sci. Mater. Med. · 1997 · 8 · 86710.1023/A:1018501804943 ↗
37. Xiao S.-J. · Langmuir · 1998 · 14 · 550710.1021/la980257z ↗
38. Gao G. · Biomacromolecules · 2011 · 12 · 371510.1021/bm2009697 ↗
39. Gao G. · Biomaterials · 2011 · 32 · 389910.1016/j.biomaterials.2011.02.013 ↗
40. Godoy-Gallardo M. · Biomacromolecules · 2014 · 16 · 48310.1021/bm501528x ↗
41. Matyjaszewski K. · Macromolecules · 1999 · 32 · 871610.1021/ma991146p ↗
42. Peltonen J. · Langmuir · 2004 · 20 · 942810.1021/la0400252 ↗
43. Gadelmawla E. S. · J. Mater. Process. Technol. · 2002 · 123 · 13310.1016/S0924-0136(02)00060-2 ↗
44. Aparicio C. · J. Mech. Behav. Biomed. Mater. · 2011 · 4 · 167210.1016/j.jmbbm.2011.05.005 ↗
45. Zhao L. · J. Biomed. Mater. Res., Part B: Appl. Biomater. · 2009 · 91 · 47010.1002/jbm.b.31463 ↗
46. Kim J. · Antimicrob. Agents Chemother. · 2008 · 52 · 144610.1128/AAC.00054-07 ↗
47. Shriver-Lake L. C. · Biosens. Bioelectron. · 1997 · 12 · 110110.1016/S0956-5663(97)00070-5 ↗
48. Chen W.-C. · Mater. Sci. Eng., C · 2013 · 33 · 271310.1016/j.msec.2013.02.040 ↗
49. Guha Thakurta S. · Colloids Surf., A: Physicochem. Eng. Aspects · 2012 · 414 · 38410.1016/j.colsurfa.2012.08.049 ↗
50. Han Y. · Thin Solid Films · 2006 · 510 · 17510.1016/j.tsf.2005.11.048 ↗
51. Bellamy W. · J. Appl. Bacteriol. · 1992 · 73 · 47210.1111/j.1365-2672.1992.tb05007.x ↗
52. Brouwer C. P. J. M. · Peptides · 2011 · 32 · 195310.1016/j.peptides.2011.07.017 ↗
53. Vermesse E. · Appl. Surf. Sci. · 2013 · 285 · 62910.1016/j.apsusc.2013.08.103 ↗
54. Rodríguez-Hernández A. · Mater. Lett. · 2013 · 92 · 4210.1016/j.matlet.2012.10.022 ↗
55. Barbour M. E. · J. Mater. Sci. Mater. Med. · 2007 · 18 · 143910.1007/s10856-007-0141-2 ↗
56. Rupp F. · Acta Biomater. · 2014 · 10 · 289410.1016/j.actbio.2014.02.040 ↗
57. Tzoneva R. · Biochim. Biophys. Acta, Gen. Subj. · 2007 · 1770 · 153810.1016/j.bbagen.2007.07.008 ↗
58. Amoroso P. F. · Clin. Oral Implants Res. · 2006 · 17 · 63310.1111/j.1600-0501.2006.01274.x ↗
59. Dexter S. C. · J. Colloid Interface Sci. · 1979 · 70 · 34610.1016/0021-9797(79)90038-9 ↗
60. Harnett E. M. · Colloids Surf., B: Biointerfaces · 2007 · 55 · 9010.1016/j.colsurfb.2006.11.021 ↗
61. Bacakova L. · Biotechnol. Adv. · 2011 · 29 · 73910.1016/j.biotechadv.2011.06.004 ↗
62. Pegueroles M. · Surf. Coat. Technol. · 2008 · 202 · 347010.1016/j.surfcoat.2007.12.021 ↗
63. Kolenbrander P. E. · Appl. Environ. Microbiol. · 1990 · 56 · 389010.1128/aem.56.12.3890-3894.1990 ↗
64. Rickard A. H. · FEMS Microbiol. Lett. · 2003 · 220 · 13310.1016/S0378-1097(03)00094-6 ↗
65. Charalampakis G. · J. Clin. Periodontol. · 2011 · 38 · 86410.1111/j.1600-051X.2011.01759.x ↗
66. Goudouri O.-M. · Acta Biomater. · 2014 · 10 · 379510.1016/j.actbio.2014.03.028 ↗
67. D’Ercole S. · J. Dent. Sci. · 2013 · 8 · 10910.1016/j.jds.2012.12.012 ↗