DataVXACS Applied Materials & InterfacesPaper
journal article·2017-09-20Open access

Injectable Carbon Nanotube-Functionalized Reverse Thermal Gel Promotes Cardiomyocytes Survival and Maturation

Brisa Peña, Susanna Bosi, Brian A. Aguado, Daniele Borin, Nikki L. Farnsworth, Evgenia Dobrinskikh, Teisha J. Rowland, Valentina Martinelli, Mark Jeong, Matthew R. G. Taylor, Carlin S. Long, Robin Shandas, Orfeo Sbaizero, Maurizio Prato, Kristi S. Anseth, Daewon Park, Luisa Mestroni

ACS Applied Materials & Interfaces· Volume 9· Issue 37
Publisher pageOpen full text
Source abstract

Abstract

The ability of the adult heart to regenerate cardiomyocytes (CMs) lost after injury is limited, generating interest in developing efficient cell-based transplantation therapies. Rigid carbon nanotubes (CNTs) scaffolds have been used to improve CMs viability, proliferation, and maturation, but they require undesirable invasive surgeries for implantation. To overcome this limitation, we developed an injectable reverse thermal gel (RTG) functionalized with CNTs (RTG-CNT) that transitions from a solution at room temperature to a three-dimensional (3D) gel-based matrix shortly after reaching body temperature. Here we show experimental evidence that this 3D RTG-CNT system supports long-term CMs survival, promotes CMs alignment and proliferation, and improves CMs function when compared with traditional two-dimensional gelatin controls and 3D plain RTG system without CNTs. Therefore, our injectable RTG-CNT system could potentially be used as a minimally invasive tool for cardiac tissue engineering efforts.
Source: openalex_inverted_index · Quality: baseline

Research context

NanomaterialsBiomaterials

OpenAlex topics: Tissue Engineering and Regenerative Medicine · Electrospun Nanofibers in Biomedical Applications · Bone Tissue Engineering Materials

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Cardiovascular Institute, University of Colorado Denver Anschutz Medical Campus, School of Medicine, Division of Cardiology, 12700 E. 19th Avenue, Bldg. P15, Aurora, Colorado 80045, United States

Department of Chemical and Biological Engineering and Howard Hughes Medical Institute and the BioFrontiers Institute, University of Colorado at Boulder, 3415 Colorado Avenue, Boulder, Colorado 80309, United States

Bioengineering Department, University of Colorado Denver Anschutz Medical Campus, Bioscience 2 1270 E. Montview Avenue, Suite 100, Aurora, Colorado 80045, United States

Department of Medicine, University of Colorado Denver Anschutz Medical Campus, 12700 E. 19th Avenue, Bldg. P15, Aurora, Colorado 80045, United States

International Center for Genetic Engineering and Biotechnology, Area Science Park, Padriciano 99, Trieste 34149, Italy

Carbon Nanobiotechnology Laboratory, CIC biomaGUNE, Paseo de Miramón 182 20009, Donostia-San Sebastián 20009, Spain

Basque Foundation for Science, Ikerbasque, Bilbao 48013, Spain

Funding

AXA Research Fund · National Heart, Lung, and Blood Institute · Ministerio de Econom?a y Competitividad · Fondazione Benefica Kathleen Foreman Casali · Burroughs Wellcome Fund · Fondation Leducq · Diputaci?n Foral Gipuzkoa · Elaine W. and Kevin P. Kauffman Family Foundation · John Patrick Albright Foundation

References (62)

Show source-provided references
1. 10.1161/CIRCRESAHA.113.30028210.1161/CIRCRESAHA.113.300282 ↗
2. 10.1016/S0735-1097(99)00172-210.1016/S0735-1097(99)00172-2 ↗
3. 10.1038/nature1173910.1038/nature11739 ↗
4. 10.1111/j.1582-4934.2008.00439.x10.1111/j.1582-4934.2008.00439.x ↗
5. Alraies M. C. · J. Thorac. Dis. · 2014 · 6 · 1120
6. 10.1093/ejcts/ezv35710.1093/ejcts/ezv357 ↗
7. 10.1002/clc.2212410.1002/clc.22124 ↗
8. 10.1016/j.jss.2014.12.03810.1016/j.jss.2014.12.038 ↗
9. 10.1089/ten.tea.2011.039110.1089/ten.tea.2011.0391 ↗
10. 10.1161/CIRCULATIONAHA.108.81269310.1161/CIRCULATIONAHA.108.812693 ↗
11. 10.1016/j.biomaterials.2014.05.01410.1016/j.biomaterials.2014.05.014 ↗
12. 10.1002/jbm.a.3478610.1002/jbm.a.34786 ↗
13. 10.1007/s10856-012-4842-910.1007/s10856-012-4842-9 ↗
14. 10.1016/j.biomaterials.2012.09.03810.1016/j.biomaterials.2012.09.038 ↗
15. 10.1073/pnas.161572811410.1073/pnas.1615728114 ↗
16. 10.1038/nmat459010.1038/nmat4590 ↗
17. 10.1016/j.jacc.2014.06.120810.1016/j.jacc.2014.06.1208 ↗
18. 10.1016/j.cjca.2015.08.02210.1016/j.cjca.2015.08.022 ↗
19. 10.1007/s10943-010-9326-y10.1007/s10943-010-9326-y ↗
20. 10.1021/acs.biomac.5b0173410.1021/acs.biomac.5b01734 ↗
21. 10.1016/j.actbio.2010.06.03910.1016/j.actbio.2010.06.039 ↗
22. 10.1007/s12265-010-9202-x10.1007/s12265-010-9202-x ↗
23. 10.1016/j.jacc.2011.10.88810.1016/j.jacc.2011.10.888 ↗
24. 10.1021/ar200162j10.1021/ar200162j ↗
25. 10.1021/la100032n10.1021/la100032n ↗
26. 10.1002/jbm.a.3534510.1002/jbm.a.35345 ↗
27. 10.1021/nn305559j10.1021/nn305559j ↗
28. 10.1021/nn503693h10.1021/nn503693h ↗
29. 10.1016/j.biotechadv.2014.05.00310.1016/j.biotechadv.2014.05.003 ↗
30. 10.1016/j.biomaterials.2012.05.03210.1016/j.biomaterials.2012.05.032 ↗
31. 10.1002/biot.20100023010.1002/biot.201000230 ↗
32. 10.1021/cn300048q10.1021/cn300048q ↗
33. 10.3389/fphys.2013.0023910.3389/fphys.2013.00239 ↗
34. 10.1021/nn400219310.1021/nn4002193 ↗
35. 10.1021/nl204064s10.1021/nl204064s ↗
36. 10.1021/acsami.6b0467910.1021/acsami.6b04679 ↗
37. 10.1080/09205063.2013.86117010.1080/09205063.2013.861170 ↗
38. Yun D. · Austin J. Biomed. Eng. · 2014 · 1 · 1
39. 10.1186/s11671-015-0972-610.1186/s11671-015-0972-6 ↗
40. 10.1002/mabi.20150006610.1002/mabi.201500066 ↗
41. 10.1002/mabi.20140025010.1002/mabi.201400250 ↗
42. Alexander G. N. · 1996
43. 10.1021/la202267p10.1021/la202267p ↗
44. 10.1039/b201013p10.1039/b201013p ↗
45. 10.1016/j.carbon.2016.11.02510.1016/j.carbon.2016.11.025 ↗
46. 10.1038/srep0373310.1038/srep03733 ↗
47. 10.1016/j.hrthm.2016.01.01110.1016/j.hrthm.2016.01.011 ↗
48. 10.1016/j.bpj.2013.11.68410.1016/j.bpj.2013.11.684 ↗
49. 10.1093/eurheartj/ehv25910.1093/eurheartj/ehv259 ↗
50. 10.1166/jnn.2007.30710.1166/jnn.2007.307 ↗
51. 10.1089/ten.teb.2009.035210.1089/ten.teb.2009.0352 ↗
52. 10.1016/j.intimp.2016.10.01910.1016/j.intimp.2016.10.019 ↗
53. 10.1093/cvr/cvn13310.1093/cvr/cvn133 ↗
54. 10.1016/j.cardiores.2003.12.00710.1016/j.cardiores.2003.12.007 ↗
55. 10.1016/j.cardiores.2003.12.01010.1016/j.cardiores.2003.12.010 ↗
56. 10.1080/19491034.2015.108445310.1080/19491034.2015.1084453 ↗
57. 10.1038/srep1338810.1038/srep13388 ↗
58. 10.1371/journal.pone.007362110.1371/journal.pone.0073621 ↗
59. 10.1016/j.arabjc.2010.08.02210.1016/j.arabjc.2010.08.022 ↗
60. 10.1515/ntrev-2012-006710.1515/ntrev-2012-0067 ↗
61. 10.1039/c1cc13011k10.1039/c1cc13011k ↗
62. 10.1016/j.addr.2016.04.02810.1016/j.addr.2016.04.028 ↗