Machine Learning-Assisted High-Throughput Strategy for Real-Time Detection of Spermine Using a Triple-Emission Ratiometric Probe

In this study, we designed and fabricated a spermine-responsive triple-emission ratiometric fluorescent probe using dual-emissive carbon nanoparticles and quantum dots, which improve the sensor's accuracy and reduce interfering environmental effects. The probe is advantageous for the proportionate detection of spermine because it has good emission resolution, and the maximum points of the two emission peaks differ by 95 nm. As a proof of concept, cuvettes and a 96-well plate were combined with a smartphone and YOLO series algorithms to accomplish real-time, visual, and high-throughput detection of seafood and meat freshness. In addition, the reaction mechanism was verified by density functional theory and fundamental characterizations. Upon exposure to different amounts of spermine, the intensity of the fluorescent probe changed linearly, and the fluorescent color shifted from yellow-green to red, with a limit of detection of 0.33 μM. To enable visual identification of food-originated spermine, a hydrogel-based visual sensing platform was successfully developed utilizing the triple-emission fluorescent probe. Consequently, spermine could be identified and quantified without complicated equipment.